Exploration of 'Over Kill Effect' of High-LET Ar-and Fe-ions by Evaluating the Fraction of Non-hit Cell and Interphase Death

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概要

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• The reason why RBE for cell killing fell to less than unity (1.0) with very high-LET heavy-ions (^<40>Ar : 1,640keV/μm; ^<56>Fe : 780, 1,200, 2,000keV/μm) was explored by evaluating the fraction of non-hit cell (time-lapse observation) and cells undergoing interphase death (calculation based on our previous data). CHO cells were exposed to 4Gy (30% survival dose) of Ar (1,640keV/μm) or Fe-ions (2,000keV/μm). About 20% of all cells were judged to be non-hit, and about 10% cells survived radiation damage. About 70% cells died after dividing at least once (reproductive death) or without dividing (interphase death). RBE for reproductive (RBE[R]) and interphase (RBE[I]) death showed a similar LET dependence with maximum around 200keV/μm. In this LET region, at 30% survival level, about 10% non-survivors underwent interphase death. The corresponding value for very high-LET Fe-ions (2,000keV/μm) was not particularly high (〜15%), whereas that for X-rays was less than 3%. However, reproductive death (67%) predominated over interphase death (33%) even in regard to rather severely damaged cells (1% survival level) after exposure to Fe-ions (2,000keV/μm). These indicate that interphase death is a type of cell death characteristic for the cells exposed to high-LET radiation and is not caused by 'cellular over kill effect'. Both NHF37 (non-hit fraction at 37% survival) and inactivation cross-section for reproductive death (σ[R]) began to increase when LET exceeded 100keV/μm. The exclusion of non-hit fraction in the calculation of surviving fraction partially prevented the fall of RBE[R] when LET exceeded 200keV/μm. On the other hand, the mean number of lethal damage per unit dose (NLD/Gy) showed the same LET-dependent pattern as RBE[R]. These suggest that the increase in non-hit fraction and σ[R] with an increasing LET is caused by enhanced clustering of ionization and DNA damage which lowers the energy efficiency for producing damage and RBE.

• 日本放射線影響学会の論文
• 2005-09-30

著者

• YATAGAI Fumio

  RIKEN Institute

• Sasaki Hiroshi

  Department of Biological Technology, Faculty of Science and Engineering,Senshu University of Ishinom

• FURUSAWA Yoshiya

  National Institute of Radiological Sciences

• FUTAMI Yasuyuki

  Shizuoka Cancer Center

• KANAI Tatsuaki

  National Institute of Radiological Science

• Futami Yasuyuki

  茨城県立医療大学 放射線技術科学科

• Futami Yasuyuki

  The Institute Of Physics University Of Tsukuba

• Futami Yasuyuki

  Dvisin Of Accelerator Physics And Engineering National Institite Of Radiological Sciences Chiba Japa

• Kanai Tatsuaki

  National Institute Of Radiological Sciences

• Kanai T

  Department Of Accelerator And Medical Physics National Institute Of Radiological Sciences

• Kanai Tatsuaki

  茨城県立医療大学 放射線技術科学科

• Kinashi Yuko

  Radiation Safety And Control Research Reactor Institute Kyoto University

• KANAI Tatsuaki

  NIRS

• YATAGAI Fumio

  IPCR

• WADA Seiichi

  Biotech. Lab., JAERI Takasaki

• KOBAYASHI Yasuhiko

  Biotech. Lab., JAERI Takasaki

• Wada Seiichi

  Department Of Veterinary Medicine Kitasato University Graduate School Of Veterinary Medicine And Ani

• Hanaoka Fumio

  Riken:graduate School Of Frontier Biosciences Osaka University

• Hanaoka Fumio

  Osaka Univ. Inst. Mol. Cell. Biol.

• Hanaoka F

  Inst. Physical And Chemical Res. Saitama Jpn

• Hanaoka Fumio

  The Institute Of Physical And Chemical Research

• KAGAWA Yasuhiro

  The Institute of Physical and Chemical Research

• Yatagai F

  Riken Saitama Jpn

• Yatagai Fumio

  Riken

• MORIMOTO Shigeko

  Div. Radioisotope Technology RIKEN

• SASAKI Hiroshi

  Dept. Med. Biophys. Radiat. Biol., Kyushu Univ.

• MEHNATI Parinaz

  Department of Medical Biophysics & Radiation Biology, Graduate School of Medical Sciences, Kyushu Un

• MORIMOTO Shigeko

  RIKEN

• Kanai T

  National Institute Of Radiological Sciences

• Kanai Tatsuaki

  Department Of Accelerator And Medical Physics Research Center For Charged Particle Therapy National

• Kanai Tatsuaki

  Department Of Medical Physics National Institute Of Radiological Sciences

• Hanaoka Fumio

  Riken &osaka Univ.

• Kamata Tadashi

  Research Center Hospital For Charged Particle Therapy National Institute Of Radiological Sciences

• Furusawa Y

  Research Center For Charged Particle Therapy National Institute Of Radiological Sciences

• Furusawa Yoshiya

  高エネルギー加速器研究機構物質構造科学研究所

• Mehnati Parinaz

  Department Of Medical Biophysics & Radiation Biology Graduate School Of Medical Sciences Kyushu

• Kanai Tatsuaki

  National Inst. Radiological Sci.

• Kanai Tatsuaki

  Div. Acce. Res. Natl. Inst. Radiol Sci.

• Kanai Tatsuaki

  Dept. Of Biochem. Gunma Univ. Graduate Sch. Med.

• Yatagai F

  Riken Institute

• Hanaoka Fumio

  Riken : Osakauniv.

• Sasaki Hiroshi

  Department Of Medical Biophysics & Radiation Biology Graduate School Of Medical Sciences Kyushu

• Furusawa Yoshiya

  Div. Of Accelerator Res. Natl. Inst. Of Radiol. Sci.

• Sasaki Hiroshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• Kanai Tatsuaki

  National Inst. Of Radiol. Sci

• HANAOKA Fumio

  RIKEN

• WADA Seiichi

  Biotech Laboratory, JAERI

• Morimoto Shigeko

  Division of Radioisotope Technology

• KOBAYASHI Yasuhiko

  Biotech Laboratory, JAERI

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