Purification and Properties of Two Endocellulases from Penicillium purpurogenum(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Two endo-type cellulases, tentatively celled carboxymethyl cellulases (CMCases) I and II, were purified by gel filtration, ion-exchange chromatography, affinity chromatography, and chromatofocusing from a culture supernatant of Penicillium Purpurogenum. Their homogeneity was verified by analytical polyacrylamide gel electrophoresis. The molecular weights of CMCases I and II, estimated by gel filtration, were 72,000 and 50,000, respectively. CMCases I and II contained about 12% and 8% carbohydrate, and had isoelectric points of 4.3 and 3.9, respectively. CMCase I produced cellobiose, glucose, and a trace amount of cellotriose from H_3PO_4-swollen cellulose and Avicel (microcrystalline cellulose), while CMCase II produced cellobiose and cellotriose with a small amount of glucose and cellotetraose. The porducts from reduced cellopentaose by both enzymes were released predominantly in the β-configuration. CMCase II appeared to act in more random fashion than I against carboxymethyl cellulose. These results suggest that both enzymes attack insoluble cellulose randomly, although there are some differences in the mode of hydrolytic action.
- 社団法人日本農芸化学会の論文
- 1986-12-23
著者
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Sasaki Hiroshi
Department of Biological Technology, Faculty of Science and Engineering,Senshu University of Ishinom
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KAMAGATA YOICHI
Department of Environmental Systems Engineering, Nagaoka University of Technology
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Yamada Kazutaka
Kankyo Engineering Corporation
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Sasaki Horoshi
Faculty of Agriculture, Tohoku University
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Takao S
Hokkaido Univ. Sapporo Jpn
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Takao Shoichi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Takao S
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Sasaki H
Tsumura Research Institute Tsumura & Co.
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Yachi Makoto
(present Address)fermentation Research Laboratories Sankyo Co. Ltd.
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YACHI Makoto
Depertment of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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Sasaki Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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