Production of Inulin Fructotransferase (Depolymerizing) by Arthrobacter sp. H65-7 and Preparation of DFA III from Inulin by the Enzyme
スポンサーリンク
概要
- 論文の詳細を見る
A bacterial strain H65-7 isolated from soil as an inulin-assimilating microorganism produces inulin fructotransferase (inulase II) which converts inulin into di-D-fructofuranose-1,2' : 2,3'-dianhydride (DFA III). This strain was classified as Arthrobacter sp. The inulase II production was induced by innulin, and markedly enhanced by the addition of yeast extract. Under optimal conditions, the enzyme activity in the culture supernatant reached 90 units/ml after cultivation for 18 h. The optimum pH and temperature for the enzyme reaction were 5.5 and 60℃, respectively. The enzyme was stable within a pH range of 4.5 to 9.0 and at up to 75℃. Using this crude enzyme, 300 mg/ml of inulin was converted into 237 mg/ml of DFA III after incubation for 4 h.
- 社団法人日本生物工学会の論文
- 1991-10-25
著者
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Hirayama S
Division Of Endocrinology And Metabolism Department Of Homeostatic Regulation And Development Niigat
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Yokota Atsushi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Tomita Fusao
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Tomita Fusao
Department Of Agricultural Biochemistry Faculty Of Agriculture Hokkaido University
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Yokota A
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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ENOMOTO KOICHI
Deparment of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University
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HIRAYAMA SATOSHI
(Present address)Novo Nordisk Bioindustry Ltd.,
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MIURA YUMIKO
(Present address)Life Technologies Oriental, Inc.
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TAKAO SHOICHI
(Present address)Department of Food Science, Rakuno Gakuen University
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Miura Yumiko
(present Address)life Technologies Oriental Inc.
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Takao S
Hokkaido Univ. Sapporo Jpn
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Enomoto Koichi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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Takao S
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
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