Production of Lepidimoide by an Endophytic Fungus from Polysaccharide Extracted from Abelmoschus sp. : Identification of the Product and the Organism Producing It

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概要

• 論文の詳細を見る

• A highly potent allelopathic factor, lepidimoide, was initially extracted from mucilage of germinated cress seeds. Polysaccharide extracted from okra (Abelmoschus esculentum Moench) is considered to have a similar structure to lepidimoide as its repeating unit. We therefore initiated the screening of enzymes capable of degrading okra polysaccharide into lepidimoide from endophytes. We discovered an endophytic fungal strain AHU9748 isolated from Coleus galeatus, which produced an oligosaccharide having similar properties to lepidimoide on thin layer chromatography. The physico-chemical data from ESI-MS, NMR spectra and other analyses also showed the purified product to be identical to lepidimoide. The strain AHU9748 was identified as a fungus belonging to the coelomycetes, closely related to the genus Colletotrichum, based on morphological characteristics and sequence analysis of the 18S rDNA and ITS region.

• 社団法人日本生物工学会の論文
• 2002-06-20

著者

• 須藤 学

  NITE・バイオ本部

• Yokota Atsushi

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• Tanaka Michiko

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• Asano Kozo

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• Tanaka Michiko

  Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University

• TOMITA FUSAO

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• YOSHIMURA MAKOTO

  Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University

• SUTO MANABU

  Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, Hokkaido University

• SUKARA ENDANG

  R & D Center for Biotechnology, LIPI

• 冨田 房男

  北海道大学農学部

• 横田 篤

  Laboratory Of Microbial Physiology Research Faculty Of Agriculture Hokkaido University

• Yokota Atsushi

  Lab. Of Microbial Physiology Res. Fac. Of Agriculture Hokkaido Univ. Kita 9 Nishi 9 Kita-ku Sapporo

• 横田 篤

  北大 大学院農学研究院

• Yokota Atsushi

  Laboratory Of Bioorganic Chemistry

• Yokota A

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Yokota Atsushi

  Hokkaido Univ. Sapporo Jpn

• Yokota Atsushi

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Asano Kozo

  Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University

• Asano Kozo

  北海道大学農学部応用生命科学専攻

• Tanaka M

  Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University

• Suto M

  Laboratory Of Microbial Resources And Ecology Graduate School Of Agriculture

• Suto Manabu

  Department Of Agricultural Biochemistry Faculty Of Agriculture Hokkaido University

• Suto Manabu

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Suto Manabu

  Lab. Microbial Resour. & Ecol. Grad. Sch. Agr. Hokkaido Univ.

• Suto Manabu

  Laboratory Of Microbial Resources And Ecology Graduate School Of Agriculture Hokkaido University

• Tomita Fusao

  Laboratory Of Applied Microbiology

• Tomita Fusao

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• Tomita Fusao

  Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok

• Sukara Endang

  R & D Center For Biotechnology Lipi

• Takebayashi M

  Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University

• Yoshimura M

  Hokkaido Univ. Sapporo Jpn

• Yoshimura Makoto

  Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University:(present Addre

• Tanaka Michiko

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Tanaka Michiko

  Laboratory Of Applied Microbiology Graduate Faculty Of Agriculture Hokkaido University

• Asano Kozo

  Laboratory Of Applied Microbiology Graduate Faculty Of Agriculture Hokkaido University

• Tanaka Michiko

  Laboratory of Applied Microbiology, Graduate Faculty of Agriculture, Hokkaido University

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