A real-time PCR method targeting a gene sequence encoding 16S rRNA processing protein, rimM, for detection and enumeration of Streptococcus thermophilus in dairy products
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概要
- 論文の詳細を見る
A real-time PCR method targeting a gene sequence encoding 16S rRNA processing protein, rimM, for specific detection of Streptococcus thermophilus was developed. The designed real-time PCR primers and probe were specific for S. thermophilus JCM20026, LMG6896, LMG18311, OJT101, OJT102 but not Enteroccocus spp., Lactococcus lactis subsp. lactis, and Streptococcus salivarius which are phylogenetically closely related to S. thermophilus and are difficult to identify using culture-based methods. The linear range of the developed real-time PCR method was from 2.7 to 8.6 log CFU ml^[-1] with an amplification efficiency of 96%. Minor differences (about 0.4 log CFU ml^[-1]) were observed between counts of S. thermophilus obtained by culture and real-time PCR method in plain yoghurt and yoghurt containing fruits. Therefore, the developed real-time PCR method could be of potential application in specific detection and accurate enumeration of S. thermophilus in a wide range of dairy products.
- Elsevier Ltd.の論文
著者
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Sone Teruo
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Sone Teruo
北海道大学 農学研究院応用生命科学部門
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Sone Teruo
Laboratory Of Applied Microbiology Research Group Of Molecular Bioscience Division Of Applied Biosci
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Tanaka Michiko
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Sone T
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Sone Teruo
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Asano Kozo
Lab. Of Applied Microbiology Graduate School Of Agriculture Hokkaido Univ. Kita-9 Nishi-9 Sapporo 06
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Asano K
Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Asano Kozo
北海道大学農学部応用生命科学専攻
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Asano Kozo
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Tanaka M
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Shiomi Takuya
Fancl Res. Inst. Fancl Corp.
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Takebayashi M
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Tanaka Michiko
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Sone Teruo
Laboratory of Applied Microbiology, Graduate Faculty of Agriculture, Hokkaido University
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Tanaka Michiko
Laboratory of Applied Microbiology, Graduate Faculty of Agriculture, Hokkaido University
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