EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae
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概要
- 論文の詳細を見る
In order to detect and enumerate DNA double-strand breaks (DSBs) during the life cycle of Magnaporthe oryzae, an expression vector for GFP-Rhm51 fusion protein was constructed and introduced into the strain Ina86-137. Rhm51-GFP foci were detected and the number of foci in mitomycin-C-treated mycelia was higher than in untreated samples, indicating that the foci visualized DSBs occurred during the life cycle. Rhm51-GFP foci were observed in all stages of the asexual life cycle including the invasive hyphae formed in an intact rice leaf sheath, demonstrating that M. oryzae suffers DSBs during vegetative and infective growth.
- Springer Japanの論文
- 2010-12-01
著者
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Sone Teruo
Laboratory Of Applied Microbiology Graduate School Of Agriculture Hokkaido University
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Sone Teruo
北海道大学 農学研究院応用生命科学部門
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Sone Teruo
Laboratory Of Applied Microbiology Research Group Of Molecular Bioscience Division Of Applied Biosci
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Sone Teruo
Research Faculty of Agriculture, Hokkaido University
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Abe Ayumi
Department Of Bioscience And Chemistry Graduate School Of Agriculture Hokkaido University
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Sone Teruo
Laboratory Of Applied Microbiology Department Of Bioscience And Chemistry Faculty Of Agriculture Hok
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Asano Kozo
Lab. Of Applied Microbiology Graduate School Of Agriculture Hokkaido Univ. Kita-9 Nishi-9 Sapporo 06
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Asano K
Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Asano Kozo
北海道大学農学部応用生命科学専攻
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Asano Kozo
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Shiomi Takuya
Fancl Res. Inst. Fancl Corp.
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Abe Ayumi
Research Faculty Of Agriculture Hokkaido University
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Asano Kozo
Research Faculty Of Agriculture Hokkaido University
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NDINDENG Sali
Graduate Faculty of Agriculture, Hokkaido University
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MIKI Shinsuke
Graduate Faculty of Agriculture, Hokkaido University
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Sone Teruo
Research Faculty Of Agriculture Hokkaido University
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Miki Shinsuke
Graduate Faculty Of Agriculture Hokkaido University
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Ndindeng Sali
Graduate Faculty Of Agriculture Hokkaido University
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Sone Teruo
Laboratory of Applied Microbiology, Graduate Faculty of Agriculture, Hokkaido University
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Abe Ayumi
Laboratory of Applied Microbiology, Graduate Faculty of Agriculture, Hokkaido University
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