Molecular Breeding of a Sake Yeast with a Mutated ARO4 Gene Which Causes Both Resistance to o-Fluoro-DL-Phenylalanine and Increased Production of β-Phenethyl Alcohol
スポンサーリンク
概要
- 論文の詳細を見る
A sake yeast, Saccharomyces cerevisiae, was transformed with a YCp vector containing a mutated ARO4 gene (ARO4-OFP) derived from S. cerevisiae which enocodes the 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase relased from feedback inhibition by tyrosine. The transformant showed feedback-insensitive DAHP synthase activity and simultaneously acquired both o-fluoro-DL-phenylalanine (OFP)-resistance and increased production of β-phenethyl alcohol and tyrosine. The greater production of the alcohol and its acetate, rose-like flavor components, improved the aroma and flavor of sake prepared experimentally with the transformant. The ARO4-OFP gene was also demonstrated to be availabel as a dominant selection marker in yeast vectors for the transformation of industrial strains of S. cerevisiae.
- 1992-05-25
著者
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Asano Kozo
北海道大学農学部応用生命科学専攻
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Asano Kozo
Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Watanabe M
Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Watanabe M
Graduate School Of Engineering Hiroshima Kokusai Gakuin University
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Ouchi K
Kyowa Hakko Kogyo Co. Ltd. Tokyo Jpn
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Ouchi Kozo
Tokyo Resarch Laboratories Kyowa Hakko Kogyo Co. Ltd.
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FUKUDA KAZURO
Foods and Liquors Research Laboratories, Kyowa Hakko Kogyo Co. Ltd.,
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WATANABE MAKOTO
Foods and Liquors Research Laboratories, Kyowa Hakko Kogyo Co. Ltd.,
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TAKASAWA SEIGO
Foods and Liquors Research Laboratories, Kyowa Hakko Kogyo Co. Ltd.,
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Takasawa S
Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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