Optimal Culture Conditions of Bacillus stearothermophilus JTS 859 for the Production of a Thermostable Enzyme to Synthesize 5-Methyluridine

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概要

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• The optimal culture conditions of a thermophile, Bacillus stearothermophilus JTS 859,were examined for the production of a thermostabel enzyme to synthesize 5-methyluridine from inosine and thymine in the presence of phosphate by the ribosyl transfer reaction. Elevation of the agitation velocity and the addition of inosine to the medium were effective in improving the production of the enzyme. By optimizing the culture conditions, the production amounts of cells and enzyme per volume of medium were increased by 4.6 and 10.1 times, respectively, compared to the initial results.

• 社団法人日本生物工学会の論文
• 1992-04-25

著者

• Hori N

  Tobacco Science Research Laboratory

• Hori Nobuaki

  Central Research Institute Japan Tobacco Inc.

• Mikami Y

  Chiba Univ. Chiba Jpn

• Watanabe M

  Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Hori Nobuaki

  Tobacco Science Research Laboratory Japan Tobacco Inc.

• WATANABE Mutsumi

  Tobacco Science Research Laboratory, Japan Tobacco Inc.

• MIKAMI Yoichi

  Tobacco Science Research Laboratory, Japan Tobacco Inc.

• Sunagawa Kenji

  Tobacco Science Research Laboratory Japan Tobacco Inc.

• Watanabe M

  Graduate School Of Engineering Hiroshima Kokusai Gakuin University

• Mikami Yoichi

  Tobacco Science Research Laboratory

• Mikami Yoichi

  Central Research Institute The Japan Tobacco 〓 Salt Public Corporation

• Hori Nobuaki

  Tobacco Science Research Laboratory

• UEHARA KYOUKO

  Tobacco Science Research Laboratory, Japan Tobacco Inc.,

• Mikami Y

  Tokyo Laboratory Yuki Gosei Kogyo Co. Ltd.

• Uehara Kyouko

  Tobacco Science Research Laboratory

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