Synthesis of 5-Methyluridine by a Thermophile, Bacillus stearothermophilus JTS 859
スポンサーリンク
概要
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Many thermophiles, which can grow at 65°C, were examined as to their ability to produce 5-methyluridine from inosine and thymine (5-methyluracil) in the presence of phosphate and cells as enzyme sources. Bacillus stearothermophilus JTS 859 was selected as a strain that synthesized 5-methyluridine efficiently. The reaction is supposed to be carried out by a combination of a thermostable purine nucleoside phosphorylase and a thermostable pyrimidine nucleoside phosphorylase. Their halflives were 7200 hr and 400 hr at 63°C, and 148 hr and 14 hr at 70°C, respectively.
- 社団法人 日本農芸化学会の論文
著者
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WATANABE Mutsumi
Tobacco Science Research Laboratory, Japan Tobacco Inc.
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YAMAZAKI Yoshinari
Tobacco Science Research Laboratory, Japan Tobacco Inc.
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Mikami Yoichi
Tobacco Science Research Laboratory
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Hori Nobuaki
Tobacco Science Research Laboratory
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HORI Nobuaki
Tobacco Science Research Laboratory, Japan Tobacco Inc.
関連論文
- Purification and Characterization of Thermostable Pyrimidine Nucleoside Phosphorylase from Bacillus stearothermophilus JTS 859(Biological Chemistry)
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