Digitoxin Biosynthesis in Isolated Mesophyll Cells and Cultured Cells of Digitalis

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概要

• 論文の詳細を見る

• In the laminae of Digitalis, most of the digitoxin present is found in the mesophyll. A new method for determining the amount of digitoxin biosynthesis using a digitoxin antibody was devised to estimate this activity in isolated mesophyll cells and cultured cells. Isolated mesophyll cells showed significant activity, which suggests that the site of biosynthesis and the accumulation of cardenolides in a lamina of Digitalis is mainly in the mesophyll cells. Of five liquid cultures of D. purpurea; green shoot-forming cultures, white shoot-forming cultures, root-forming cultures, undifferentiated green cells and undifferentiated white cells, the green shoot-forming cultures had the highest activity. The white shoot-forming cultures had about one-third the activity of the green shoot-forming cultures, and the other three cultures had very low activity. No stimulatory effect of light was found during the 48-h incubation.

• 日本植物生理学会の論文

著者

• MATSUMOTO Takashi

  Central Research Institute, Japan Tobacco Inc.

• Mikami Y

  Chiba Univ. Chiba Jpn

• Mikami Yoichi

  Tobacco Science Research Laboratory

• Mikami Yoichi

  Central Research Institute The Japan Tobacco 〓 Salt Public Corporation

• Matsumoto T

  Faculty Of Integrated Arts And Sciences The University Of Tokushima

• Mikami Y

  Tokyo Laboratory Yuki Gosei Kogyo Co. Ltd.

• Hagimori Manabu

  Central Research Institute, The Japan Tobacco & Salt Public Corporation

• Hagimori M

  Japan Tobacco Inc. Tochigi Jpn

• Hagimori Manabu

  Central Research Institute Japan Tobacco Inc.

• Matsumoto Takashi

  Central Research Institute Japan Tobacco Inc.

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