Jar Fermenter Culture of Shoot-forming Cultures of Digitalis purpurea L. Using a Revised Medium
スポンサーリンク
概要
- 論文の詳細を見る
A revised medium for digitoxin production by shoot-forming cultures of Digitalis purpurea L. was established. Using this medium, jar fermenter culture of the shoot-forming cultures was performed. Approximately 25 g fresh weight of cells was inoculated into a 3-liter jar fermenter containing 2.5 liter of the medium, and cultured for 15 days at 28°C with aeration of 1.4vvm without mechanical agitation. The jar was illuminated continuously throughout the culture period. The cell yield was 38.5 g dry weight perjar and the total digitoxin formation was 1610 μg per jar. The optimumrate of aeration was 1.4vvm and mechanical agitation had little effect.
- 社団法人 日本農芸化学会の論文
著者
-
Mikami Yoichi
Central Research Institute The Japan Tobacco 〓 Salt Public Corporation
-
Hagimori Manabu
Central Research Institute Japan Tobacco Inc.
-
MIKAMI Yoichi
Central Research Institute, The Japan Tobacco & Salt Public Corporation
-
MATSUMOTO Takashi
The Japan Tobacco & Salt Public Corporation
関連論文
- Purification and Characterization of Thermostable Pyrimidine Nucleoside Phosphorylase from Bacillus stearothermophilus JTS 859(Biological Chemistry)
- Purification and Characterization of Second Thermostable Purine Nucleoside Phosphorylase in Bacillus stearothermophilus JTS 859(Biological Chemistry)
- Purification and Characterization of Thermostable Purine Nucleoside Phosphorylase of Bacillus stearothermophilus JTS 859(Biological Chemistry)
- Efficient Production of 2-Deoxyribose 5-Phosphate from Glucose and Acetaldehyde by Coupling of the Alcoholic Fermentation System of Baker's Yeast and Deoxyriboaldolase-Expressing Escherichia coli
- Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2'-Deoxyribonucleosides(Microbiology & Fermentation Technology)
- Optimal Culture Conditions of Bacillus stearothermophilus JTS 859 for the Production of a Thermostable Enzyme to Synthesize 5-Methyluridine
- Microbial Conversion of β-Myrcene by Aspergillus niger(Microbiology & Fermentation Industry)
- Microbial Conversion of 4-Oxoisophorone by Aspergillus niger(Microbiology & Fermentation Industry)
- Purification and Some Properties of an Exo-β-1,3-Glucanase from Porodisculus pendulus
- Purification and Some Properties of an Endo-β-1,3-Glucanase from Porodisculus pendulus
- Molecular Weight-Viscosity Relationships of Pendulan from Porodisculus pendulus
- Change in Molecular Weight of Pendulan from Porodisculus pendulus
- Rheological Properties of Pendulan from Porodisculus pendulus
- Bulk Preparation of R-(+)-Nicotine from a Racemic Mixture by Continuous Culture with an Isolated Pseudomonas putida
- Studies on the Production of Digitalis Cardenolides by Plant Tissue Culture III. : Effects of Nutrients on Digitoxin Formation by Shoot-Forming Cultures of Digitalis purpurea L. Grown in Liquid Media
- Enzymatic Synthesis of 5-Methyluridine from Adenosine and Thymine with High Efficiency
- Effects of Xanthine Oxidase on Synthesis of 5-Methyluridine by the Ribosyl Transfer Reaction(Microbiology & Fermentation Industry)
- Photoautotrophic Culture of Undifferentiated Cells and Shoot-Forming Cultures of Digitalis purpurea L.
- Digitoxin Biosynthesis in Isolated Mesophyll Cells and Cultured Cells of Digitalis
- Purification and Characterization of Acid Invertase from Cultured Tobacco Cells(Biological Chemistry)
- Studies on the production of Digitalis cardenolides by plant tissue culture I. Determination of digitoxin and digoxin contents in first and second passage calli and organ redifferentiating calli of several Digitalis species by radioimmunoassay
- Effects of Mineral Salts, Initial pH and Precursors on Digitoxin Formation by Shoot-forming Cultures of Digitalis purpurea L. Grown in Liquid Media
- Preparation of Aroma Compounds by Microbial Transformation of Isophorone with Aspergillus niger
- Jar Fermenter Culture of Shoot-forming Cultures of Digitalis purpurea L. Using a Revised Medium
- Formation of (2S, 6R, 7R)-2, 7-Epoxydihydro-α-ionone via Asymmetric Hydroxylation of β-Ionone by Aspergillus niger
- Effects of Physical Factors and Surface Active Agents on the Formation of Anthraquinones by Rubia cordifolia Cells in Suspension Culture
- Effects of Nutritional Factors on the Formation of Anthraquinones by Rubia cordifolia Plant Cells in Suspension Culturel