Effects of Mineral Salts, Initial pH and Precursors on Digitoxin Formation by Shoot-forming Cultures of Digitalis purpurea L. Grown in Liquid Media
スポンサーリンク
概要
- 論文の詳細を見る
Shoot-forming cultures of Digitalis purpurea L. were grown in various modifications of Murashige-Skoog medium. The effects of CaCl2, MgSO4, FeSO4 coupled with Na2-EDTA, minor elements, initial pH of the medium, some organic solvents as precursor solvents, and precursors on their growth and digitoxin formation were investigated. Reduction of the basal CaCl2 concentration by two-thirds promoted both digitoxin formation and shoot-differentiation while a threefold increase repressed these. Ca++ proved to be responsible for this phenomenon. A threefold increase in the MgSO4 concentration improved digitoxin formation, Mg++ being responsible for this improvement. The optimal concentration of FeSO4 coupled with Na2-EDTA was similar to that in the MS basal medium. A threefold increase in the concentration of either KI, Na2MoO4 or CuSO4 improved digitoxin formation, the optimal pH of the medium before autoclaving being 6.Among methanol, ethanol, propylene glycol, dimethyl sulfoxide and acetone as the precursor solvents, methanol and acetone were relatively the least inhibitive at 2%. Progesterone at 0.1mg/100ml medium improved the digitoxin content per flask to 180% of that of the control cultures, whereas no promotive effect from cholesterol was observed.
- 社団法人 日本農芸化学会の論文
著者
-
Obi Yukiteru
Central Research Institute The Japan Tobacco & Salt Public Corporation
-
Hagimori Manabu
Central Research Institute Japan Tobacco Inc.
-
Matsumoto Takashi
Central Research Institute Japan Tobacco Inc.
関連論文
- Effects of Culture Conditions on the Formation of Anti-plant-viral Protein by Highly Producing Cell Strains of Mirabilis jalapa L.(Biological Chemistry)
- Selection of Cultured Mirabilis jalapa L. Cell Strains Producing High Levels of Anti-plant-viral Protein(Biological Chemistry)
- Effects of Nutritional and Hormonal Factors on the Formation of Anti-plant-viral Protein by Mirabilis jalapa L. Cells in Suspension Culture(Biological Chemistry)
- Effects of Phosphate Concentration on Growth in Mirabilis jalapa Cultured Cells
- Selection of Cultured Tobacco Cell Strains Producing High Levels of Ubiquinone 10 by a Cell Cloning Technique
- Changes in Endogenous Ribosyl-trans-zeatin and IAA Levels in Relation to the Proliferation of Tobacco Crown Gall Cells
- Relationship between hormone content and autonomy in various autonomous tobacco cells cultured m suspension
- Studies on the Production of Digitalis Cardenolides by Plant Tissue Culture III. : Effects of Nutrients on Digitoxin Formation by Shoot-Forming Cultures of Digitalis purpurea L. Grown in Liquid Media
- Photoautotrophic Culture of Undifferentiated Cells and Shoot-Forming Cultures of Digitalis purpurea L.
- Digitoxin Biosynthesis in Isolated Mesophyll Cells and Cultured Cells of Digitalis
- Purification and Characterization of Acid Invertase from Cultured Tobacco Cells(Biological Chemistry)
- Studies on the production of Digitalis cardenolides by plant tissue culture I. Determination of digitoxin and digoxin contents in first and second passage calli and organ redifferentiating calli of several Digitalis species by radioimmunoassay
- An Enzyme-Linked Immunosorbent Assay for Desmosine
- Characteristics of Cultured Tobacco Cell Strains Producing High Levels of Ubiquinone-10 Selected by a Cell Cloning Technique
- Effects of Mineral Salts, Initial pH and Precursors on Digitoxin Formation by Shoot-forming Cultures of Digitalis purpurea L. Grown in Liquid Media
- Preparation of Aroma Compounds by Microbial Transformation of Isophorone with Aspergillus niger
- Jar Fermenter Culture of Shoot-forming Cultures of Digitalis purpurea L. Using a Revised Medium
- Influences of Cultural Conditions on Polyphenol Formation and Growth of Amacha Cells (Hydrangea macrophylla Seringe var. Thunbergii Makino) and Changes of Polyphenol Contents in Leaves of Amacha Plant during Growth
- Formation of (2S, 6R, 7R)-2, 7-Epoxydihydro-α-ionone via Asymmetric Hydroxylation of β-Ionone by Aspergillus niger
- Mutagen from the Gaseous Phase of Protein Pyrolyzate
- Influences of Copper Concentration on Cytochrome aa3 Formation and Growth in Cultured Tobacco Cells
- Synthesis and Mutagenic Activity of Alkyl Derivatives of 2-Amino-9H-pyrido[2, 3-b]indole
- Effects of Nutritional Factors on the Formation of Anthraquinones by Rubia cordifolia Plant Cells in Suspension Culturel