Relationship between hormone content and autonomy in various autonomous tobacco cells cultured m suspension

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概要

• 論文の詳細を見る

• Various autonomous cultured tobacco cells including crown gall were examined for their contents of growth regulators by means of Avena curvature test, cell-division induction test, and tobacco pith callus test. The crown gall cells derived from cv. Hicks produced auxin and cytokinin in the high levels of 300-500μg IAA equivalents and 40-80μg kinetin equivalents per kg, respectively. The major auxin was identified as indole-3-acetic acid based on mass spectrometry and gas chromatography. These cells also produced methyl indole-3-acetate as a minor component. One of the cytokinins was identified as ribosyl-trans-zeatin by means of both gas chromatography-mass spectrometry and high performance liquid chromatography. Auxin and cytokinin activities were not detected in the following three suspension-cultured tobacco cells : cells requiring neither cytokinin nor auxin derived from the callus of N. tabacum cv. Bright Yellow and cells requiring auxin but not cytokinin derived from the calluses of cv. Bright Yellow and cv. Hicks. Their auxin and cytokinin contents per kg were less than 1μg IAA equivalent and less than 0.1μg kinetin equivalent, respectively. The results obtained in this study indicate that enhanced hormonal content is not the only reason for autonomous growth.

• 日本植物生理学会の論文

著者

• TAKAHASHI Nobutaka

  Department of Biochemistry University of Shizuoka School of Pharmaceutical Science

• MATSUMOTO Takashi

  Central Research Institute, Japan Tobacco Inc.

• NOGUCHI Masao

  Central Research Institute, The Japan Tobacco & Salt Public Corporation

• 高橋 直樹

  Univ. Tokyo Tokyo Jpn

• YOKOTA TAKAO

  Department of Pediatrics, The Second Tokyo National Hospital

• Takahashi Nobutaka

  Department Of Agricultural Chemistry The University Of Takyo

• Nakajima Hiromitsu

  Department of Bioresource Science, Faculty of Agriculture, Tottori University

• Yokota Takao

  Department Of Agricultural Chemistry The University Of Takyo

• Noguchi Masao

  Central Research Institute The Japan Tobacco Public Corporation

• Noguchi Masao

  Central Research Institute The Japan Tobacco & Salt Public Corporation

• Yokota T

  Teikyo Univ. Tochigi Jpn

• Nakajima Hiromitsu

  Department Of Agricultural Chemistry The University Of Takyo:(present)department Of Agricultural Che

• Nakajima Hiromitsu

  Department Of Agricultural Chemistry Faculty Of Agriculture Tottori University

• Matsumoto Takashi

  Central Research Institute The Japan Tobacco Public Corporation

• Matsumoto Takashi

  Central Research Institute Japan Tobacco Inc.

• Takahashi Nobutaka

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

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