Selection of Cultured Tobacco Cell Strains Producing High Levels of Ubiquinone 10 by a Cell Cloning Technique

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概要

• 論文の詳細を見る

• Strains producing high levels of ubiquinone 10 were isolated from tobacco cell suspension cultures by a cell cloning technique, and the UQ content of these cell strains was analyzed by high performance liquid chromatography. Three strains with high UQ yields in suspension culture were selected and used for investigations on the necessary cultural conditions for UQ production. Sucrose concentration, 2, 4-d concentration and culture temperature did not have marked effects on UQ formation. These results differed from those of the original cell lines. Furthermore, the addition of yeast extract to the medium promoted cell growth in these strains remarkably but did not enhance UQ production in the cell strains. According to these investigations, UQ production was increased to 15mg/liter and the UQ level to 1890μg/g dry wt. of cells after 11 days of incubation.

• 社団法人 日本農芸化学会の論文

著者

• Ikeda T

  Central Research Institute Japan Tobacco Inc.

• IKEDA Tsutomu

  Central Research Institute, Japan Tobacco Inc.

• MATSUMOTO Takashi

  Central Research Institute, Japan Tobacco Inc.

• KANNO Naomi

  Central Research Institute, The Japan Tobacco & Salt Public Corporation

• OBI Yukiteru

  Central Research Institute, The Japan Tobacco & Salt Public Corporation

• KISAKI Takuro

  Central Research Institute, The Japan Tobacco & Salt Public Corporation

• NOGUCHI Masao

  Central Research Institute, The Japan Tobacco & Salt Public Corporation

• Obi Yukiteru

  Central Research Institute The Japan Tobacco & Salt Public Corporation

• Kisaki Takuro

  Central Research Institute Japan Monopoly Corporation

• Noguchi Masao

  Central Research Institute The Japan Tobacco & Salt Public Corporation

• Matsumoto Takashi

  Central Research Institute Japan Tobacco Inc.

• NOGUCHI Masao

  Central Research Institute, The Japan Tobacco & Salt Public Corporation

• IKEDA Tsutomu

  Central Research Institute, The Japan Tobacco & Salt Public Corporation

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