Electrocatalysis with a Glucose-Oxidase-immobilized Graphite Electrode
スポンサーリンク
概要
- 論文の詳細を見る
Glucose oxidase was immobilized on the surface of a graphite electrode by irreversible adsorption. Anelectrocatalytic steady-state current for the oxidation of D-glucose was observed using this electrode in the presence of p-benzoquinone as an electron transfer mediator. The electrocatalytic current at 0.5 V vs. SCEwas analyzed as a function of the concentrations of dglucose and p-benzoquinone, and the maximum current, Imaxs, and the Michaelis constants (K1 and K2 for D-glucose and p-benzoquinone, respectively) of the electrocatalysis were determined. The dependence of the current on the electrode potential, pH, and temperature was also investigated. The results indicate that the kinetics of the immobilized enzyme are essentially the same as those of the enzyme in the solubilized state. The effect of various electron transfer mediators on the electrocatalytic current was also examined and evaluated in terms of Imaxs, K1, and K2 values.
- 社団法人 日本農芸化学会の論文
著者
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Senda Mitsugi
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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Ikeda T
Central Research Institute Japan Tobacco Inc.
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Ikeda Tokuji
Department Of Bioscience Fukui Prefectural University
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KATASHO Isao
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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KAMEI Masugu
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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Ikeda Tokuji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Senda Mitsugi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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