Electrocatalytic Oxidation of D-Gluconate at a Ubiquinone-Mixed Carbon Paste Electrode with an Immobilized Layer of D-Gluconate Dehydrogenase from Bacterial Membranes(Analytical Chemistry)

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概要

• 論文の詳細を見る

• D-Gluconate dehydrogenase (EC. 1.1.99.3) from pseudomonas fluorescens was immobilized on the surface of a ubiquinone-mixed carbon paste electrode by irreversible adsorption. This electrode could oxidize D-gluconate electrocatalytically, with ubiquinone serving as an electron transfer mediator between the carbon paste electrode and the immobilized enzyme. The catalytic oxidation current observed with this electrode was analyzed as a function of the concentration of D-gluconate, the amount of mixed ubiquinone in the carbon paste electrode and the potential applied to the electrode. The results were explained by the theory of the catalytic current at an enzyme-modified electrode with an electron transfer mediator. The parameters characterizing the current response of the electrode to D-gluconate were determined.

• 社団法人日本農芸化学会の論文
• 1987-03-22

著者

• Senda Mitsugi

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• Ikeda T

  Central Research Institute Japan Tobacco Inc.

• Ikeda Tokuji

  Department Of Bioscience Fukui Prefectural University

• FUSHIMI Fumiyoshi

  Department of Agricultural Chemistry, Kyoto University

• MIKI Koujiro

  Department of Agricultural Chemistry, Kyoto University

• Ikeda Tokuji

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• Senda Mitsugi

  Department Of Agricultural Chemistry College Of Agriculture Kyoto University

• Senda M

  Department Of Bioscience Fukui Prefectural University

• Miki Koujiro

  Department Of Agricultural Chemistry Kyoto University

• Fushimi Fumiyoshi

  Department Of Agricultural Chemistry Kyoto University

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