Effect of temperature on the polarographic catalytic current produced by proteins in the presence of cobalt salts.
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概要
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The effect of temperature on the polarographic catalytic hydrogen evolution current (Brdicka current) produced by ribonuclease-A in an ammoniacal buffer containing cobalt salt has been studied using the theoretical equation of Brdi\`{c}ka current. With increasing temperature, the Brdicka current at −1.3 V increased between 10 and 30 °C then fell off above 30 °C, whereas the currents at −1.4 and −1.5 V decreased continuously. Analysis showed that the initial increase of the Brdicka current at −1.3 V was only apparent and that the Brdicka current constant, <I>k</I><SUB>B</SUB>, which represents the catalytic activity of the protein, always decreased with increasing temperature in the potential range of −1.3 to −1.5 V. Further analysis has revealed that the intrinsic catalytic activity of the protein-cobalt(0) complex, which is transiently formed on the electrode surface and which catalyzes the hydrogen evolution, increases with increasing temperature whereas the life time of the complex decreases and the latter decrease cancels the former increase, resulting in the decrease of the <I>k</I><SUB>B</SUB> value with increasing temperature.
- 公益社団法人 日本化学会の論文
著者
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Kano Kenji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Ikeda Tokuji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Senda Mitsugi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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Tokimitsu Ichiro
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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