Electrophoresis, Zeta potential and Surface Charges of Barley Mesophyll Protoplasts

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概要

• 論文の詳細を見る

• The electrophoretic mobility of barley mesophyll protoplasts was measured by the micro-electrophoresis technique to determine the surface charge density in medium that contained various cations and anions at neutral pH. The surface charge was strongly influenced by the presence of di- and trivalent cations in the medium, suggesting that certain di- and trivalent cations are specifically adsorbed on or bound to the plasma membrane of the protoplasts. The order of ad-sorbability was Mg^<2+> < Ca^<2+>, Mn^<2+>, Ba^<2+> for divalent cations, and La^<3+> < < Sm^<3+> < Tm^<3+>. Yb^<3+> for trivalent cations. The adsorption of Ca^<2+> and La^<3+> ions on the surface of the membrane was analyzed using the Langmuir adsorption isotherm. The maximum amount of adsorption and the adsorption coefficient were found to be 3.17 mC/m^2 and 1.16 mM^<-1> for Ca^<2+> ions, and 7.12 mC/ m^2 and 8.26 mM^<-1> for La^<3+> ions, respectively.

• 日本植物生理学会の論文

著者

• OBI Ichiro

  Division of Applied Life Science, Graduate School of Agriculture, Kyoto University

• KAKUTANI Tadaaki

  Division of Applied Life Science, Graduate School of Agriculture, Kyoto University

• Obi Ichiro

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• Kakutani Tadaaki

  Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University

• Ichikawa Yoshiaki

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• Senda Mitsugi

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• Obi I

  Kyoto Univ. Kyoto Jpn

• KAKUTANI Tadaaki

  Kyoto Univ.

• Kakutani Tadaaki

  Division Of Applied Life Science Graduate School Of Agriculture Kyoto University

• Kakutani Tadaaki

  Research Center For Cell And Tissue Culture Faculty Of Agriculture Kyoto University

• Ichikawa Yoshiaki

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• Senda Mitsugi

  Department Of Agricultural Chemistry College Of Agriculture Kyoto University

• Senda M

  Department Of Bioscience Fukui Prefectural University

• Ichikawa Y

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• Obi Ichiro

  Department of Agricultural Chemistry and Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University

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