Polarographic Catalytic Current of Microbial Protein Proteinase Inhibitors and Direct Determination of Dissociation Constants of Subtilisin BPN-Inhibitor Complexes by Polarographic Method
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概要
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Brdicka current (polarographic catalytic hydrogenevolution current produced by proteins in the presence of cobalt salt) of Streptomyces subtilisin inhibitor (SSI) and plasminostreptin (PS) was studied with dme and hmde. The Brdicka current of the inhibitors decreased with addition of subtilisin BPN (S.BPN), which was attributed to the formation of proteinase-inhibitor complexes. Analysis of the dependence of Brdicka current of SSI and S.BPN-SSI complex on cobalt ion concentration revealed that one of two SS bonds of SSI becamenon-accessible to Brdicka reaction by the formation of proteinase-inhibitor complexes. SSI and PS could be titrated by polarographic method using Brdicka current. The titration curves were analyzed by a multiple equilibrium equation of complex formation. The polarographic method was proved useful for determining the dissociation constants of S.BPN-inhibitor complexes as low as 10-10 M.
- 社団法人 日本農芸化学会の論文
著者
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Senda Mitsugi
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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Kano K
Kyoto Univ. Kyoto Jpn
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Ikeda Tokuji
Department Of Bioscience Fukui Prefectural University
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KANO Kenji
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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Kano Kenji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Ikeda Tokuji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Senda Mitsugi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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