Differential Pulse Polarographic Determination of the First and Second Dissociation Constants of Dimeric Subtilisin EPN-Streptomyces Subtilisin Inhibitor Complex
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概要
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A differential pulse polarographic method based on the Brdicka current (polarographic catalytic hydrogen evolution current produced by proteins in the presence of the cobalt ion) was applied to the direct titration of subtilisin BPN (S.BPN) with Streptomyces subtilisin inhibitor(SSI) at a concentration level of 10-9 M. The first and second dissociation constants of the dimeric S.BPN-SSI complex were determined by fitting theoretical curves to the titration data, in which the multiple equilibrium involving microscopically distinct forms of the S.BPN-SSI complex was taken into account. The intrinsic free energy change in the first binding of S.BPN to dimeric SSI was larger than that of the second binding.
- 社団法人 日本農芸化学会の論文
著者
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Senda Mitsugi
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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Kano K
Kyoto Univ. Kyoto Jpn
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Ikeda Tokuji
Department Of Bioscience Fukui Prefectural University
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Ikeda T
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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KANO Kenji
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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Kano Kenji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Ikeda Tokuji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Senda Mitsugi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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