Membrane Potential of Cultured Carrot Cells in Relation to the Synthesis of Anthocyanin and Embryogenesis

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Membrane potentials of cultured carrot cells in culture medium were about -40 mV and did not change with addition of salts of addition (or depletion) of 2,4-dichlorophenoxyacetic acid (2,4-D). When the measurement was performed in the test medium (containing low concentration of salts), the values were widely distributed (from -60 to - 110 mV) and changed largely with external concentration of K^+ but not Mg^<2+> nor Ca^<2+>. When the cells were fractionated by Ficoll density gradient centrifugation, the membrane potential of the cells of higher density (> 14% Ficoll) was about -150 mV in the test medium and did not change during embryogenesis with depletion of 2,4-D. The membrane potential of cells of lower density (banding between 6-10% Ficoll) was less negative (-60 to -110 mV) in the test medium. When these cells were transferred and cultured in medium containing zeatin but lacking 2,4-D, the membrane potential was shifted negatively by about 15 mV prior to anthocyanin synthesis. When 2,4-D was added to anthocyanin-synthesizing cells in the medium containing zeatin, a transient hyperpolarization and subsequent depolarization of the membrane were observed prior to the inhibition of anthocyanin synthesis.
日本植物生理学会の論文