Expression of Mangrove eEF1A Enhances Tolerance to Salt and Osmotic Stress in Escherichia coli

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概要

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• To analyze the mechanisms of salt tolerance in the mangrove plant, Bruguiera sexangula, functional screening for mangrove cDNAs encoding proteins essential for salt tolerance was performed using Escherichia coli as the host organism. A transformant expressing a eukaryotic elongation factor 1A homologue (Bs-eEF1A) displayed enhanced tolerance to salt and osmotic stress. This distinct function was not conferred by other plant eEF1A homologues isolated from two halophytes (Suaeda japonica and Salsola komarovii) or Arabidopsis thaliana. Levels of Bs-eEF1A transcripts in cultured B. sexangula cells were enhanced in response to salt stress. These data suggest that Bs-eEF1A plays an important role in the salt-tolerance mechanisms of B. sexangula, in addition to its original function in elongation during translation.

• 2003-03-03

著者

• OZEKI Yoshihiro

  Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agricultur

• MIMURA Tetsuro

  Department of Biology, Graduate School of Science, Kobe University

• Mimura T

  Nara Women's Univ. Nara Jpn

• Mimura Tetsuro

  Department Of Biological Science Faculty Of Science Nara-women's University

• Nozaki Asami

  Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

• Nozaki Asami

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• YAMADA Akiyo

  Department of Biotechnology, Tokyo University of Agriculture and Technology

• Ozeki Yoshihiro

  Department Of Biology College Of Arts And Science The University Of Tokyo

• Yamada Akiyo

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• SANO Emiko

  Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

• SANO Emiko

  Dept. Biotechnol., TUAT, Tokyo

• Sano Emiko

  Department Of Biotechnology Faculty Of Technology Tokyo University Of Agriculture And Technology

• Yamada Akiyo

  Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

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