Furosemide : a Specific Inhibitor of P_i Transport across the Plasma Membrane of Plant Cells : MEMBRANES AND BIOENERGETICS

元データ 日本植物生理学会

概要

A search was made for inhibitors of P_i uptake that act directly on the P_i transporter in the plasma membranes of Catharanthus roseus cells to inhibit P_i uptake without inhibition of proton pumping. Using standard electrodes, we monitored changes in pH and in the concentration of K^+ ions, as well as the rate of P_i uptake, when an inhibitor to be tested was applied to the cells in unbuffered medium. A9C (28 μM), a blocker of anion channels, inhibited P_i uptake but it also inhibited the proton pump. However, a structurally similar inhibitor, furosemide, inhibited P_i uptake without inhibiting proton pumping. It is suggested that the carboxylic group of these inhibitors interacts with the P_i-binding site (probably an amino group) of the P_i transporter in the plasma membrane and that the hydrophobic structure of these inhibitors facilitates their accumulation in the plasma membrane.

著者

MIMURA Tetsuro Department of Biology, Graduate School of Science, Kobe University
Mimura Tetsuro Department Of Biological Science Faculty Of Science Nara-women's University
Mimura Tetsuro Department Of Life Science Himeji Institute Of Technology:(present)department Of Biology Hitotsubash
Sakano Katsuhiro Department Of Applied Physiology National Institute Of Agrobiological Resources
Kiyota Seiichiro Department Of Agricultural Chemistry Chiba University
Kiyota Seiichiro Department Of Applied Physiology National Institute Of Agrobiological Resources
OKIHARA Kiyoshi Department of Applied Physiology, National Institute of Agrobiological Resources
Okihara Kiyoshi Department Of Applied Physiology National Institute Of Agrobiological Resources

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