Furosemide : a Specific Inhibitor of P_i Transport across the Plasma Membrane of Plant Cells : MEMBRANES AND BIOENERGETICS

概要

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A search was made for inhibitors of P_i uptake that act directly on the P_i transporter in the plasma membranes of Catharanthus roseus cells to inhibit P_i uptake without inhibition of proton pumping. Using standard electrodes, we monitored changes in pH and in the concentration of K^+ ions, as well as the rate of P_i uptake, when an inhibitor to be tested was applied to the cells in unbuffered medium. A9C (28 μM), a blocker of anion channels, inhibited P_i uptake but it also inhibited the proton pump. However, a structurally similar inhibitor, furosemide, inhibited P_i uptake without inhibiting proton pumping. It is suggested that the carboxylic group of these inhibitors interacts with the P_i-binding site (probably an amino group) of the P_i transporter in the plasma membrane and that the hydrophobic structure of these inhibitors facilitates their accumulation in the plasma membrane.
日本植物生理学会の論文