Vacuolar Function in the Phosphate Homeostasis of the Yeast Saccharomyces cerevisiae
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概要
- 論文の詳細を見る
We studied physiological roles of the yeast vacuole in the phosphate metabolism using ^<31>P-in vivo nuclear magnetic resonance (NMR) spectroscopy. Under phosphate starvation wild-type yeast cells continued to grow for two to three generations, implying that wild-type cells contain large phosphate pool to sustain the growth. During the first four hours under the phosphate starved condition, the cytosolic phosphate level was maintained almost constant, while the vacuolar pool of phosphate decreased significantly. ^<31>P-NMR spectroscopy on the intact cells and perchloric acid (PCA) extracts showed that drastic decrease of poly-phosphate took place during this phase. In contrast, Δslp1 cells, which were defective in the vacuolar compartment, thus lacked polyphosphate, ceased their growth immediately when they faced to phosphate starvation. Taken together, we conclude that vacuolar polyphosphate provides an active pool for phosphate and is mobilized to cytosol during phosphate starvation and sustained cell growth for a couple rounds of cell cycle.
- 日本植物生理学会の論文
著者
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Wada Yoh
Department of Biology, Graduate School of Arts and Sciences, University of Tokyo
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Yazaki Yoshiaki
Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University
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Ohsumi Yoshinori
Department Of Biology College Of Arts And Sciences University Of Tokyo
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Sakano Katsuhiro
Department Of Applied Physiology National Institute Of Agrobiological Resources
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Yazaki Y
National Inst. Agrobiological Resources Tsukuba Jpn
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Yazaki Yoshiaki
Department Of Applied Physiology National Institute Of Agrobiological Resources
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Ohsumi Y
National Inst. For Basic Biology Okazaki Jpn
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Wada Yoh
Department Of Biology Graduate School Of Arts And Sciences University Of Tokyo
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Wada Yoh
Department Of Biology College Of Arts And Sciences University Of Tokyo
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Shirahama Kanae
Department Of Cell Biology National Institute For Basic Biology Department Oflife Sciences Graduate
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Shirahama Kanae
Dapartment of Biology, Graduate School of Arts and Sciences, University of Tokyo
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Wada Yoh
Dapartment of Biology, Graduate School of Arts and Sciences, University of Tokyo
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Ohsumi Yoshinori
Dapartment of Biology, Graduate School of Arts and Sciences, University of Tokyo
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Shirahama Kanae
Department Of Cell Biology National Institute For Basic Biology Department Oflife Sciences Graduate
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