Acidification of Vacuoles Is Required for Autophagic Degradation in the Yeast, Saccharomyces cerevisiae.
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概要
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Acidification inside vacuoles has been shown to play a key role in a number of physiologi-cally important cellular events. We studied the role of vacuolar membrane H+-ATPase in the autophagic process of Saccharomyces cerevisiae. Mutants lacking VMA genes which encode their subunits of the vacuolarH+-ATPase accumulated autophagic bodies in vacuoles on starvation. vma mutants also had a defect in protein degradation induced by starvation. In vma mutants, the activities of vacuolar proteases were remarkably lower than those of the wild-type. Overexpression of vacuolar proteases did not overcome the defect in the disintegration of autophagic bodies in vma mutant, even the overexpressed proteinase A and proteinase B being substantially localized to the vacuolar compartment and undergoing proper proteolytic maturation. Our results showed that the acidification of vacuoles is not required for the formation and delivery of autophagosomes to vacuoles, but is essential for the disintegration of autophagic bodies.
- 社団法人 日本生化学会の論文
著者
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Ohsumi Yoshinori
Department Of Biology College Of Arts And Sciences University Of Tokyo
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Wada Yoh
Department Of Biology College Of Arts And Sciences University Of Tokyo
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Matsuura Akira
Department Of Biology Faculty Of Science Chiba University
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Wada Yoh
Department of Biology, Graduate School of Arts and Sciences, The University of Tokyo
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Nakamura Norihiro
Department of Biological Science, Graduate School of Science, Osaka University
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