Regulation of Cytoplasmic pH under Extreme Acid Conditions in Suspension Cultured Cells of Catharanthus roseus : a Possible Role of Inorganic Phosphate

元データ 日本植物生理学会

概要

Changes in cytoplasmic pH of suspension-cultured cells of Catharanthus roseus under extreme acid conditions were measured with the pH-dependent fluorescence dye;2', 7'-bis-(2-carboxyethyl)-5(and-6)carboxfluorescein(-acetoxymethylester)(BCECF). When cells were treated with 1 mM HCl(pH 3 solution), the cytoplasmic pH first decreased then returned to the original level. Treatment with 10 mM HCl(pH 2 solution)acidified the cytoplasm to a greater extent, and the acidification continued at a constant level throughout the measurement. Treatment with a pH 2 solution resulted in a gradual decrease of the malate content, indicating the operation of biochemical pH regulation mechanism. The pH 2 treatment also caused a sudden decrease of the intracellular level of Pi. The cellular content of total phosphorus did not change during the acidification. The Pi was converted to the organic phosphate form. The ATP level was not increased by the pH 2 treatment, but slightly decreased. The role of Pi, which might be functioning as a regulatory factor of cytoplasmic pH, a non-competitive inhibitor of the H^+-pumps of both the plasma membrane and tonoplast is discussed.

著者

加藤 美砂子 お茶の水女子大
新免 輝男 Department Of Life Science Faculty Of Science Himeji Institute Of Technology
Mimura T Hitotsubashi Univ. Tokyo Jpn
Mimura T Himeji Inst. Technology Hyogo Jpn
Shimmen Teruo Department of Life Science, Faculty of Science, Himeji Institute of Technology
加藤 美砂子 東大教養生物
Ashihara Hiroshi Department of Biology, Faculty of Science, Ochanomizu University
Ashihara Hiroshi Department Of Advanced Biosciences Graduate School Of Humanities And Sciences Ochanomizu University
YOKOTA Etsuo Department of Life Science, Graduate School of Life Science, University of Hyogo
Mimura Tetsuro Biological Laboratory Hitotsubashi University
Yokota E Himeji Inst. Of Technol. Hyogo Jpn
Yokota Etsuo Department Of Life Science Faculty Of Science Himeji Institute Of Technology
Sakano K Department Of Applied Physiology National Institute Of Agrobiological Resources
Sakano Katsuhiro Department Of Applied Physiology National Institute Of Agrobiological Resources
Shimmen Teruo Department Of Biology Faculty Of Science University Of Tokyo
Kato Misako Department of Biology, University of Tokyo
Shindo Chihiro Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park
KATO Misako Dept. Biol., Ochanomizu Univ.
Kato Misako Department Of Biology Faculty Of Science Ochanomizu University
Kato Misako Graduate School Of Humanities And Sciences Ochanomizu University
Kato Misako Marine Biotechnology Institute Shimizulab.
Kato Misako Department Of Molecular Biology And Biochemistry Graduate School Of Lite Sciences Ochanomizu Univers
Shindo Chihiro Department Of Life Science Faculty Of Science Himeji Institute Of Technology Harima Science Park Cit
Shimmen Teruo Department Of Biology Faculty Of Science Osaka University:(present)department Of Botany Faculty Of S
Mimura Tetsuro Biological Labolatory Hitotsubashi University

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