Studies on Mechano-Perception in Characeae : Effects of External Ca^<2+> and Cl^-

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The effects of modification of extracellular concentrations of Ca^<2+> and Cl^- on mechano-perception were studied in internodal cells of Chara corallina. Cells were stimulated by dropping a piece of glass tubing on them, and the resulting receptor potentials and action potentials were analyzed. When the Ca^<2+> concentration was extremely lowered by adding EGTA, the amplitudes of both receptor potentials and action potentials were attenuated, suggesting the involvement of Ca^<2+> channels. However, the possibility remained that attenuation of the amplitude of the receptor potential was caused by modification of membrane characteristics by extreme lowering of [Ca^<2+>]_0. When the plasma membrane was depolarized to about O mV by adding 100 mM KCl, responses in the negative direction were induced upon mechanical stimulation. When the plasma membrane was depolarized by adding 50 mM K_2SO_4, responses in the positive direction were induced. Thus, Cl^- channels may be involved in responses induced by mechanical stimulation under K^+-induced depolarization.
日本植物生理学会の論文