Plant Microtubules can be Translocated by a Dynein ATPase from Sea Urchin in Vitro : PROTEINS, ENZYMES AND METABOLISM: STRUCTURE AND FUNCTION OF CELLS

元データ 日本植物生理学会

概要

The latent ability of plant microtubules to translocate or slide in the presence of motor protein was examined in a motility assay in vitro. Plant microtubules isolated from tobacco BY-2 cells moved over a glass surface covered with outer arm 21S dynein from flagella of sea urchin sperm with an average velocity of 3.7 μm s^<-1^gt;. This velocity was similar to that of microtubules isolated from bovine brain under the same conditions (average velocity, about 4.1 μm s^<-1^gt;). These results suggest that plant microtubules have an intrinsic ability to interact with and to be translocated by dynein. It is postulated that microtubule-based motor proteins, including dynein ATPase, are involved in the functioning of microtubules in plant cells.

著者

新免 輝男 Department Of Life Science Faculty Of Science Himeji Institute Of Technology
Sonobe S Graduate School Of Life Science University Of Hyogo
Sonobe Seiji Department Of Biology Faculty Of Science Osaka University
YOKOTA Etsuo Department of Life Science, Graduate School of Life Science, University of Hyogo
Igarashi Hisako Plant Science Center Riken
Yokota Etsuo Department Of Life Science Graduate School Of Life Science University Of Hyogo
Yokota Etsuo Department Of Life Science Faculty Of Science Himeji Institute Of Technology
Shimmen Teruo Department Of Biology Faculty Of Science University Of Tokyo
Igarashi Hisako Department Of Life Science Graduate School Of Life Science University Of Hyogo
Shimmen Teruo Department Of Biology Faculty Of Science Osaka University:(present)department Of Botany Faculty Of S

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