Association of Microtubules with the Plasma Membrane of Tobacco BY-2 Cells in Vitro : STRUCTURE AND FUNCTION OF CELLS

概要

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Membrane ghosts prepared from tobacco BY-2 cells bound microtubules (MTs) that had been assembled in an extract of evacuolated protoplasts of the same cells and, as a result, the number of MTS On the ghosts increased. MTS that had formed on the ghosts after incubation with the extract were more sensitive to cold-treatment than the preexisting MTs. Binding of MTS was also observed on ghosts that had been treated with a cold solution of Ca^<2+> to remove preexisting MTs. This result suggests that a system that mediated the binding of MTS to the plasma membrane remained on the ghosts. No binding of MTS was observed to ghosts that had been treated with trypsin. Preexisting MTS were removed by treatment with 0.6 M KCl or 0.1 M Na_2CO_3. Membrane ghosts treated with KCl or Na_2CO_3 did not bind MTS that had been assembled in the extract in the absence of taxol. However, ghosts prepared in the same way did bind MTS that had been assembled in the extract in the presence of taxol. It seems that treatment with KCl or Na_2CO_3 removes a component(s) from the system that mediates the binding of MTS to the plasma membrane and that a factor(s), associated with MTS assembled in the extract in the presence of taxol, compensates for the loss of this component(s) from the system.
日本植物生理学会の論文