Patch-Clamp Study on Ion Channels in the Tonoplast of Nitellopsis obtusa
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概要
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Cytoplasmic drops covered with the tonoplast were prepared from internodal cells of Nitellopsis grown in fresh water. Applying the patch-clamp technique and the microinjection technique to such drops, we characterized the ion channels in the tonoplast. Both in cell-free patches and in the cytoplasmic-drop-attached patches, the tonoplast K^+ channel was identified. The permeability ratio between Na^+ and K^+ was calculated to be 0.2. This channel would provide a molecular basis for the Na^+/K^+ exchange at the tonoplast. In cell-free patches, the K^+ channel was not activated by Ca^<2+>. However, in the case of attached patches, microinjection of Ca^<2+> into a drop activated the K^+ channel with a lag of a few seconds, suggesting that some cytoplasmic factor(s) may mediate the activation of the K^+ channel by Ca^<2+>. The conductance of this channel was not changed by cytoplasmic Ca^<2+> , but the probability of opening increased markedly. In addition to the K^+ channel, a second type of channel was also identified in cell-free patches. This channel may be the Cl^-channel.
- 日本植物生理学会の論文
著者
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Mimura T
Hitotsubashi Univ. Tokyo Jpn
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Mimura T
Himeji Inst. Technology Hyogo Jpn
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Mimura Tetsuro
Department Of Biological Science Faculty Of Science Nara-women's University
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KATSUHARA Maki
Research Institute for Bioresources, Okayama University
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Katsuhara M
Research Institute For Bioresources Okayama University
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Katsuhara Maki
Department Of Insect Physiology And Behavior National Institute Of Sericultural And Entomological Sc
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Tazawa M
Fukui Univ. Technol. Fukui Jpn
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Tazawa Masashi
Department Of Applied Physics And Chemistry Fukui University Of Technology
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Tazawa Masashi
Departlnent Of Biology Faculty Of Science Osaka University
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