Cyioplasmic Hydration Triggers a Transient Increase in Cyioplasmic Ca^<2+> Concentration in Nitella flexilis : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
スポンサーリンク
概要
- 論文の詳細を見る
Cells of Nitella flexilis were made inexcitable by treatment with 10 mM KCl for more than 24 h. A Ca^<2^+>-sensitive photoprotein aequorin was injected into the cytoplasm of such cells. For vacuolar perfusion, the central part of an aequorin-loaded cell was immersed in silicone oil, and both cell ends bathed in the perfusion medium were cut off. A large light emission from aequorin was observed when the vacuole was perfused with a hypotonic medium whose osmotic pressure was adjusted to half of the osmotic pressure of the cell sap. This shows that hydration of the cytoplasm triggers release of Ca^<2^+> from internal stores, since influx of Ca^<2^+> from silicone oil is excluded. Hydration of cells was induced in another way. Cells were first dehydrated by transferring them from 10 mM KCl solution to that with 250 mM sorbitol added. This procedure did not affect the cytoplasmic streaming. When cells were rehydrated by transferring them to 10 mM KCl solution, cytoplasmic streaming was either stopped or slowed down in a few seconds. A quick light emission from aequorin was observed in the rehydration, evidence that cytoplasmic streaming was inhibited by an increase in the cytoplasmic Ca^<2^+> concentration. Both streaming cessation and aequorin light emission were observed even in KCl-treated cells which were further treated with 5 mM EGTA. Thus, the increase in Ca^<2^+> is concluded to be caused by the release of Ca^<2^+> from internal stores. These results support our previous hypothesis [Tazawa et al. (1994) Plant Cell Physiol. 35: 63] that, in Nitella flexilis, the increase in the concentration of Ca^<2^+> in the cytoplasm which occurs on the endoosmotic side of the cell during transcellular osmosis is caused by hydration of the cytoplasm.
- 日本植物生理学会の論文
著者
-
Kikuyama Munehiro
Biological Laboratory, The University of the Air
-
Kikuyama M
Niigata Univ. Niigata Jpn
-
Shimada K
Tokyo Metropolitan Univ. Tokyo Jpn
-
Shimada Kiyo
Biological Laboratory The University Of The Air
-
Tazawa M
Fukui Univ. Technol. Fukui Jpn
-
Tazawa Masashi
Department Of Applied Physics And Chemistry Fukui University Of Technology
-
Kikuyama Munehiro
Biological Laboratory The University Of The Air.
-
Kikuyama Munehiro
Biological Laboratory The University Of The Air
-
Tazawa Masashi
Departlnent Of Biology Faculty Of Science Osaka University
関連論文
- Role of Ca^ on Triggering Tonoplast Action Potential in Intact Nitella flexilis
- Turgor Regulation in a Brackish Water Charophyte, Lamprothamnium succinctum III. Changes in Cytoplasmic Free Amino Acids and Sucrose Contents during Turgor Regulation
- Different Cytoplasmic Calcium Contents Among Three Species of Characeae
- Contribution of Calcium Influx on Trichocyst Discharge in Paramecium caudatum(Cell Biology)(Origin and evolution of chordates)
- CYTOPLASMIC CALCIUM CONTENT IN CHARACEAE
- REGULATION OF ION FLUXES BY PROTEIN PHOSPHORYLATION IN OLIGOSACCHARIDE ELICITOR SIGNALING IN RICE.
- Membrane Depolarization Induced by N-Acetylchitooligosaccharide Elicitor in Suspension-Cultured Rice Cells
- The Hydration-Induced Ca^ Release Is a General Phenomenon in Characeae
- MEMBRANE RESPONSES INDUCED BY AN ELICITOR ON CULTURED RICE CELLS.
- Membrane Control of Cytoplasmic Streaming in Characean Cells
- Studies on Cessation of Cytoplasmic Streaming under K^+-Induced Depolarization in Nitella axilliformis
- Cyioplasmic Hydration Triggers a Transient Increase in Cyioplasmic Ca^ Concentration in Nitella flexilis : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- MOBILIZATION OF CA^ DURINGHYDRATION OF CYTOPLASMIN CHARACEAE
- VACUOLE IS NOT INVOLVED INHYDRATION-INDUCED CA^-RELEASE IN NITELLA
- Characean Cells as a Tool for Studying Electrophysiological Characteristics of Plant Cells
- Intracellular Mobilization of Ca^ and Inhibition of Cytoplasmic Streaming Induced by Transcellular Osmosis in Internodal Cells of Nitella flexilis : MEMBARANES AND BIOENERGETICS : STRUCTURE AND FUNCTION OF CELLS
- Potassium and Chloride Effluxes during Excitation of Characeae Cells
- Relationship between light-induced potential change and internal ATP concentration in tonoplast-free Chara cells
- Demonstration of light-induced potential change in Chara cells lacking tonoplast
- PHOTOSYNTHETIC GENES IN ACIDOPHILIC BACTERIA, Acidiphilium
- Action Spectrum of Light-Induced Membrane Hyperpolarization in Egeria densa
- Respiration-Dependent Membrane Hyperpolarization in Tonoplast-Free Cells of Nitella axilliformis
- Induction of Cytoplasmic Streaming and Movement of Chloroplast Induced by L-Histidine and its Derivatives in Leaves of Egeria densa
- Excitation and Emission Spectroscopy of Membranes and Pigment-protein Complexes of an Aerobic Photosynthetic Bacterium, Erythrobacter sp. OCh 114
- Intercellular Transport of Macromolecules in Nitella
- Mercurial-Sensitive Water Transport in Barley Roots
- CONTINUOUS MEASUREMENT OF TURGOR PRESSURE WITH TURGOR BALANCE METHOD AIDED BY COMPUTER IMAGE ANALYSIS
- CHARACTERISTICS OF WATER CHANNELS IN CHARAINHIBITION BY MERCURY AND ZINC
- CHARACTERISTICS OF WATER CHANNELS INCHARA II
- Light-Induced Changes in Cytosolic pH in Leaf Cells of Egeria densa : Measurements with pH-Sensitive Microelectrodes : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Effect of intracellular pH on the light-induced potential change and electrogenic activity in tonoplast-free cells of Chara australis
- Light-induced changes in membrane potential in Spirogyra
- Dependence of Plasmalemma Conductance and Potential on Intracellular Free Ca^ in Tonoplast-Removed Cells of a Brackish Water Characeae Lamprothamnium
- Turgor Regulation in a Brackish Charophyte, Lamprothamnium succinctum : II. Changes in K^+, Na^+ and Cl^- Concentrations, Membrane Potential and Membrane Resistance during Turgor Regulation
- Turgor Regulation in a Brackish Charophyte, Lamprothamnium succinctum : I. Artificial Modification of Intracellular Osmotic Pressure
- Cytoplasmic Alkalization and Cytoplasmic Streaming Induced by Light and Histidine in Leaf Cells of Egeria densa: in vivo ^P-NMR study
- Patch-Clamp Study on Ion Channels in the Tonoplast of Nitellopsis obtusa
- Patch-Clamp Study on a Ca^-Regulated K^+ Channel in the Tonoplast of the Brackish Characeae Lamprothamnium succinctum
- Temporal Relationship between Action Potential and Ca^ Transient in Characean Cells
- STUDIES ON NITELLA HAVING ARTIFICIAL CELL SAP II. RATE OF CYCLOSIS AND ELECTRIC POTENTIAL
- Cessation of cytoplasmic streaming of Chara internodes during action potential
- Is Ca^ Release from Internal Stores Involved in Membrane Excitation in Characean Cells?
- STUDIES ON NITELLA HAVING ARTIFICIAL CELL SAP I. REPLACEMENT OF THE CELL SAP WITH ARTIFICIAL SOLUTIONS
- Intracellular Distribution of Free Amino Acids between the Vacuolar and Extravacuolar Compartments in Internodal Cells of Chara australis
- Potassium, sodium and Chloride in the protoplasm of characeae
- Mechanosensitive Ca^ Release from Intracellular Stores in Nitella flexilis
- Open-vacuole method for measuring membrane potential and membrane resistance of Characeae cells
- Involvement of Water Channels in the Hydration-Induced Calcium Release in Nitella flexilis
- Activation of K^+-Channel in Membrane Excitation of Nitella axilliformis
- Effect of osmotic shock on auxin-induced cell extension, cell wall changes and acidification in Avena coleoptile segments
- Light-Induced Membrane Hyperpolarization and Adenine Nucleotide Levels in Perfused Characean Cells
- Jumping Transmission of Action Potential between Separately Placed Internodal Cells of Chara corallina
- Analysis of Rapid Light-Induced Potential Change in Cells of Chara corallina
- Effects of Intracellular Vanadate on Electrogenesis, Excitability and Cytoplasmic Streaming in Nitellopsis obtusa
- Ionic Activity Gradients across the Surface Membrane of Cytoplasmic Droplets Prepared from Chara australis
- Demonstration of Voltage Dependency of Light-induced Potential Change in Chara
- Dependency of H^+ efflux on ATP in cells of Chara australis
- Influence of Cytoplasmic Streaming and Turgor Pressure Gradient on the Transnodal Transport of Rubidium and Electrical Conductance in Chara corallina
- Uptake and Utilization of Sugars in Cultured Rice Cells
- WATER PERMEABILITY OF THE CELL WALL IN NITELLA
- CHANGES IN RESTING POTENTIAL AND ION ABSORPTION INDUCED BY LIGHT IN A SINGLE PLANT CELL
- Effect of Cytoplasmic Ca_ on the Membrane Potential and Membrane Resistance of Chara Plasmalemma
- Action Potential in Luffa cylindlica and its Effects on Elongation Growth
- Cytoplasmic Ca_ has No Effect on the Excitability of Chara Plasmalemma
- Quantitative Analysis of ATP-Dependent H^+ Efflux and Pump Current Driven by an Electrogenic Pump in Nitellopsis obtusa
- Electrogenic Pump Current and ATP-Dependent H^+ Efflux Across the Plasma Membrane of Nitellopsis obtusa
- Onion Root Water Transport Sensitive to Water Channel and K^+ Channel Inhibitors
- Turgor Pressure Regulation and the Orientation of Cortical Microtubules in Spirogyra Cells
- Rapid light-induced potential change in Chara cells stained with neutral red in the absence of internal Mg-ATP
- Osmotic and ionic regulation in Nitella
- Demonstration of the K^+ channel in the plasmalemma of tonoplast-free cells of Chara australis
- Direct demonstration of the involvement of chloroplasts in the rapid light-induced potential change in tonoplast-free cells of Chara australis. Replacement of Chara chloroplasts with spinach chloroplasts
- Plant plasma membrane H^+ pumps : past and present
- Cell physiological aspects of the plasma membrane electrogenic H^+ pump
- Purification of Actin Based Motor Protein from Chara corallina.
- In vitro Demonstration of Hydration-Induced Calcium Release in Cells of Nitella flexilis.