Direct demonstration of the involvement of chloroplasts in the rapid light-induced potential change in tonoplast-free cells of Chara australis. Replacement of Chara chloroplasts with spinach chloroplasts

元データ 日本植物生理学会

概要

To directly demonstrate the involvement of chloroplasts in the rapid light-induced potential change we removed chloroplasts by centrifuging tonoplast-free cells of Chara australis. Chloroplast-free cells showed no signs of a potential change on illumination. When a chloroplast-free Chara cell was perfused with a suspension of chloroplasts isolated from other cells of Chara australis, it regained its ability to generate the rapid potential change induced by light. Chloroplasts were isolated from spinach leaves by a method which preserves the chloroplast envelope; thus ensuring the ability to assimilate CO_2. When these chloroplasts were introduced into a transparent Chara cell containing no chloroplasts, normal rapid hyperpolarization was induced on illumination. When spinach chloroplasts were treated with a medium of very low osmotic potential, no O_2 evolution was detected, but even with these chloroplasts rapid light-induced hyperpolarization was observed. We concluded that chloroplasts are essential for the rapid light-induced potential change and that chloroplasts can be replaced with those of another species, so far as the light-induced potential change is concerned.

著者

Tazawa Masashi Departlnent Of Biology Faculty Of Science Osaka University
Shimmmen Teruo Department of Botany, Faculty of Science, University of Tokyo
Shimmmen Teruo Department Of Botany Faculty Of Science University Of Tokyo

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