Direct demonstration of the involvement of chloroplasts in the rapid light-induced potential change in tonoplast-free cells of Chara australis. Replacement of Chara chloroplasts with spinach chloroplasts
スポンサーリンク
概要
- 論文の詳細を見る
To directly demonstrate the involvement of chloroplasts in the rapid light-induced potential change we removed chloroplasts by centrifuging tonoplast-free cells of Chara australis. Chloroplast-free cells showed no signs of a potential change on illumination. When a chloroplast-free Chara cell was perfused with a suspension of chloroplasts isolated from other cells of Chara australis, it regained its ability to generate the rapid potential change induced by light. Chloroplasts were isolated from spinach leaves by a method which preserves the chloroplast envelope; thus ensuring the ability to assimilate CO_2. When these chloroplasts were introduced into a transparent Chara cell containing no chloroplasts, normal rapid hyperpolarization was induced on illumination. When spinach chloroplasts were treated with a medium of very low osmotic potential, no O_2 evolution was detected, but even with these chloroplasts rapid light-induced hyperpolarization was observed. We concluded that chloroplasts are essential for the rapid light-induced potential change and that chloroplasts can be replaced with those of another species, so far as the light-induced potential change is concerned.
- 日本植物生理学会の論文
著者
-
Tazawa Masashi
Departlnent Of Biology Faculty Of Science Osaka University
-
Shimmmen Teruo
Department of Botany, Faculty of Science, University of Tokyo
-
Shimmmen Teruo
Department Of Botany Faculty Of Science University Of Tokyo
関連論文
- Turgor Regulation in a Brackish Water Charophyte, Lamprothamnium succinctum III. Changes in Cytoplasmic Free Amino Acids and Sucrose Contents during Turgor Regulation
- Different Cytoplasmic Calcium Contents Among Three Species of Characeae
- CYTOPLASMIC CALCIUM CONTENT IN CHARACEAE
- The Hydration-Induced Ca^ Release Is a General Phenomenon in Characeae
- Membrane Control of Cytoplasmic Streaming in Characean Cells
- Studies on Cessation of Cytoplasmic Streaming under K^+-Induced Depolarization in Nitella axilliformis
- Cyioplasmic Hydration Triggers a Transient Increase in Cyioplasmic Ca^ Concentration in Nitella flexilis : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Characean Cells as a Tool for Studying Electrophysiological Characteristics of Plant Cells
- Potassium and Chloride Effluxes during Excitation of Characeae Cells
- Relationship between light-induced potential change and internal ATP concentration in tonoplast-free Chara cells
- Demonstration of light-induced potential change in Chara cells lacking tonoplast
- Action Spectrum of Light-Induced Membrane Hyperpolarization in Egeria densa
- Respiration-Dependent Membrane Hyperpolarization in Tonoplast-Free Cells of Nitella axilliformis
- Induction of Cytoplasmic Streaming and Movement of Chloroplast Induced by L-Histidine and its Derivatives in Leaves of Egeria densa
- Mercurial-Sensitive Water Transport in Barley Roots
- Light-Induced Changes in Cytosolic pH in Leaf Cells of Egeria densa : Measurements with pH-Sensitive Microelectrodes : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Effect of intracellular pH on the light-induced potential change and electrogenic activity in tonoplast-free cells of Chara australis
- Light-induced changes in membrane potential in Spirogyra
- Dependence of Plasmalemma Conductance and Potential on Intracellular Free Ca^ in Tonoplast-Removed Cells of a Brackish Water Characeae Lamprothamnium
- Turgor Regulation in a Brackish Charophyte, Lamprothamnium succinctum : II. Changes in K^+, Na^+ and Cl^- Concentrations, Membrane Potential and Membrane Resistance during Turgor Regulation
- Turgor Regulation in a Brackish Charophyte, Lamprothamnium succinctum : I. Artificial Modification of Intracellular Osmotic Pressure
- Cytoplasmic Alkalization and Cytoplasmic Streaming Induced by Light and Histidine in Leaf Cells of Egeria densa: in vivo ^P-NMR study
- Patch-Clamp Study on Ion Channels in the Tonoplast of Nitellopsis obtusa
- Patch-Clamp Study on a Ca^-Regulated K^+ Channel in the Tonoplast of the Brackish Characeae Lamprothamnium succinctum
- Temporal Relationship between Action Potential and Ca^ Transient in Characean Cells
- STUDIES ON NITELLA HAVING ARTIFICIAL CELL SAP II. RATE OF CYCLOSIS AND ELECTRIC POTENTIAL
- Cessation of cytoplasmic streaming of Chara internodes during action potential
- Is Ca^ Release from Internal Stores Involved in Membrane Excitation in Characean Cells?
- STUDIES ON NITELLA HAVING ARTIFICIAL CELL SAP I. REPLACEMENT OF THE CELL SAP WITH ARTIFICIAL SOLUTIONS
- Intracellular Distribution of Free Amino Acids between the Vacuolar and Extravacuolar Compartments in Internodal Cells of Chara australis
- Potassium, sodium and Chloride in the protoplasm of characeae
- Mechanosensitive Ca^ Release from Intracellular Stores in Nitella flexilis
- Open-vacuole method for measuring membrane potential and membrane resistance of Characeae cells
- Involvement of Water Channels in the Hydration-Induced Calcium Release in Nitella flexilis
- Activation of K^+-Channel in Membrane Excitation of Nitella axilliformis
- Effect of osmotic shock on auxin-induced cell extension, cell wall changes and acidification in Avena coleoptile segments
- Light-Induced Membrane Hyperpolarization and Adenine Nucleotide Levels in Perfused Characean Cells
- Jumping Transmission of Action Potential between Separately Placed Internodal Cells of Chara corallina
- Analysis of Rapid Light-Induced Potential Change in Cells of Chara corallina
- Effects of Intracellular Vanadate on Electrogenesis, Excitability and Cytoplasmic Streaming in Nitellopsis obtusa
- Ionic Activity Gradients across the Surface Membrane of Cytoplasmic Droplets Prepared from Chara australis
- Demonstration of Voltage Dependency of Light-induced Potential Change in Chara
- Dependency of H^+ efflux on ATP in cells of Chara australis
- Influence of Cytoplasmic Streaming and Turgor Pressure Gradient on the Transnodal Transport of Rubidium and Electrical Conductance in Chara corallina
- Uptake and Utilization of Sugars in Cultured Rice Cells
- WATER PERMEABILITY OF THE CELL WALL IN NITELLA
- CHANGES IN RESTING POTENTIAL AND ION ABSORPTION INDUCED BY LIGHT IN A SINGLE PLANT CELL
- Effect of Cytoplasmic Ca_ on the Membrane Potential and Membrane Resistance of Chara Plasmalemma
- Action Potential in Luffa cylindlica and its Effects on Elongation Growth
- Cytoplasmic Ca_ has No Effect on the Excitability of Chara Plasmalemma
- Quantitative Analysis of ATP-Dependent H^+ Efflux and Pump Current Driven by an Electrogenic Pump in Nitellopsis obtusa
- Electrogenic Pump Current and ATP-Dependent H^+ Efflux Across the Plasma Membrane of Nitellopsis obtusa
- Onion Root Water Transport Sensitive to Water Channel and K^+ Channel Inhibitors
- Turgor Pressure Regulation and the Orientation of Cortical Microtubules in Spirogyra Cells
- Rapid light-induced potential change in Chara cells stained with neutral red in the absence of internal Mg-ATP
- Osmotic and ionic regulation in Nitella
- Demonstration of the K^+ channel in the plasmalemma of tonoplast-free cells of Chara australis
- Direct demonstration of the involvement of chloroplasts in the rapid light-induced potential change in tonoplast-free cells of Chara australis. Replacement of Chara chloroplasts with spinach chloroplasts
- Plant plasma membrane H^+ pumps : past and present
- Cell physiological aspects of the plasma membrane electrogenic H^+ pump
- In vitro Demonstration of Hydration-Induced Calcium Release in Cells of Nitella flexilis.