Cytoplasmic Alkalization and Cytoplasmic Streaming Induced by Light and Histidine in Leaf Cells of Egeria densa: in vivo ^P-NMR study

元データ 日本植物生理学会


Rotational streaming of the cytoplasm including chloroplasts was induced by L-histidine, as well as by light, on the anticlinal face of leaf cells of Egeria densa. In the case of treatment with L-histidine some of the chloroplasts remained stationary on the periclinal face of cells after rotational cytoplasmic streaming was initiated. However, these chloroplasts were easily dislodged and translocated to the centrifugal end of the histidine-treated cells by application of a centrifugal force that barely affected the location of chloroplasts in cells incubated in the dark without L-histidine. This result indicates that the anchoring of chloroplasts was weakened by L-histidine. Thus only the release of chloroplasts from anchoring was not enough for initiation of their streaming. The cytoplasmic pH (pH_c) and vacuolar pH (pH_v) were noninvasively monitored by in vivo <31>^P-nuclear magnetic resonance (NMR) spectroscopy. Compared with the dark control value, both illumination and treatment with L-histidine increased the pH_c by 0.3 units. In contrast, pH_v changed only a little with both illumination and treatment with L-histidine. Release of chloroplast from anchoring and initiation of cyloplasmic streaming are discussed in relation to the increase in pH_c, induced by both light and L-histidine.


Miyachi S Marine Biotechnology Inst. Kamaishi Lab. Iwate Jpn
KATSUHARA Maki Research Institute for Bioresources, Okayama University
Miyachi Shigetoh Institute of Applied Microbiology, University of Tokyo
Kuchitsu Kazuyuki Dept. Biotech. Natl. Inst. Agrobiol. Resources
Katsuhara M Research Institute For Bioresources Okayama University
Katsuhara Maki Department Of Insect Physiology And Behavior National Institute Of Sericultural And Entomological Sc
Tazawa Masashi Department Of Applied Physics And Chemistry Fukui University Of Technology
Miyachi Sigetoh Marine Biotechnology Institute Kamaishi Laboratories
Tominaga Yoshito St. Agnes' Junior College
Kuchitsu Kazuyuki Institute of Applied Microbiology, University of Tokyo
Miyachi Shigetoh Institute Of Applied Microbiology Univcrsity Of Tokyo
Tazawa Masashi Departlnent Of Biology Faculty Of Science Osaka University
Tominaga Yoshito St. Agnes' Junior College
Miyachi Shigetoh Institutc Of Applied Microbiology University Of Tokyo