QUANTITATIVE SEPARATION OF INORGANIC POLYPHOSPHATES IN CHLORELLA CELLS
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概要
- 論文の詳細を見る
Using unicellular green alga, Chlorella ellipsoidea, which had been labeled with ^<32>P-phosphate, re-examination was performed on our routine method for the quantitative separation of inorganic polyphosphates (poly-Pi). Confirming the previous results, it was demonstrated that poly-Pi's in Chlorella cells are clearly separated from each other by successive extractions with cold 8% TCA (poly-Pi "A"), with cold KOH at pH 9 (poly Pi "B") and with 2_N KOH (poly Pi's "C" and "D"; the former precipitates on neutralization with PCA, Ieaving the latter in solution). Use of 2_N KOH was found to be most suitable for the purpose of fractionation of poly-Pi's "C" and "D ".
- 日本植物生理学会の論文
著者
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Miyachi Shigetoh
Institute of Applied Microbiology, University of Tokyo
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Kanai Ryuzi
Institute Of Applied Microbiology University Of Tokyo:the Tokugawa Institute For Biological Research
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Aoki Shigeji
Institute Of Applied Microbiology University Of Tokyo:the Tokugawa Institute For Biological Research
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Aoki Shigeji
Institute Cf Applied Microbiology University Of Tokyo And Tokugawa Institute For Biological Research
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Miyachi Shigetoh
Institute Of Applied Microbiology Univcrsity Of Tokyo
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Miyachi Shigetoh
Institute Of Applied Microbiology University Of Tokyo:the Tokugawa Institute For Biological Research
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Miyachi Shigetoh
Institutc Of Applied Microbiology University Of Tokyo
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