DISTRIBUTION AND TURNOVER OF PHOSPHATE COMPOUNDS IN GROWING CHLORELLA CELLS

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1. Using the Chlorella cells which had been uniformly labeled with ^<32>P, the distribution of phosphorus in various fractions of cell material was investigated. Uniformly ^<32>P-labeled Chlorella was further grown in a P-free medium or in a standard "cold" medium, and the change of distribution of ^<32>P (as well as the uptake of exogenous P) in various cell fractions was followed. 2. Analysis of the ^<32>P-labeled algal cells showed that the highest in P-content was the fraction of RNA followed by those of polyphosphates, lipid, nucleotidic labile phosphate compounds, DNA and protein (in decreasing order). ATP and ADP were found to be only minor fractions of the total labile phosphates. 3. On incubating the ^<32>P-labeled alga in a P-free medium, the P-contents in the fractions of DNA, protein, lipid and ATP increased, those in polyphosphates and ADP decreased, and that in RNA remained almost unchanged. When the ^<32>P-labeled alga was further grown in the normal "cold" medium, DNA and protein increased with the expenditure of endogenous ^<32>P, but with practically no incorporation of external P. In the meantime the ^<32>P in polyphosphates decreased considerably, and the RNA fraction incorporated a large amount of external P but only a little of endogenous ^<32>P. 4. It was inferred that, under the experimental conditions of the present study, the phosphorus used in the syntheses of DNA and protein was primarily taken from polyphosphates, while that used in the syntheses of RNA, phospholipid and polyphosphates was, for the most part, taken from the extracellular P-source.
日本植物生理学会の論文

DISTRIBUTION AND TURNOVER OF PHOSPHATE COMPOUNDS IN GROWING CHLORELLA CELLS

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