Effects of dark preincubation and chloramphenicol on blue light-induced CO_2 incorporation in Chlorella cells

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1) Chlorella cells incubated in the dark longer than 12 hr showed pronounced blue light-induced ^<14>CO_2 fixation into aspartate, glutamate, malate and fumarate (blue light effect), whereas those kept under continuous light showed only a slight blue light effect, if any. 2) During dark incubation of Chlorella cells, phosphoenolpyruvate carboxylase activity and the capacity for dark ^<14>CO_2 fixation decreased significantly, whereas ribulose-1,5-diphosphate carboxylase activity and the capacity For photosynthetic ^<14>CO_2 fixation (measured under illumination of white light at a high light intensity) did not decrease. 3) In cells preincubated in the dark, intracellular levels of phosphoenolpyruvate and 3-phosphoglycerate determined during illumination with blue light were practically equal to levels determined during illumination with red light. 4)The blue light effect was not observed in cells incubated with chloramphenicol, indicating that blue light-induced protein synthesis is involved in the mechanism of the effect.
日本植物生理学会の論文