CHROMATOGRAPHIC ANALYSES OF ACID-SOLUBLE POLYPHOSPHATES IN CHLORELLA CELLS
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概要
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1. Using uniformly ^<32>P-labeled Chlorella cells as material, composition of acid-soluble inorganic polyphosphates was studied by paper chromatography and ion-exchange chromatography. 2. By the paper chromatographic analysis it was found that the acid-soluble polyphosphates consisted of highly condensed polyphosphates. Ring-forming try- and tetrametaphosphates, pyrophosphate and tripolyphosphate were not detected in the acid-soluble fraction of the algal cells. 3. By an ion-exchange chromatography with the use of increasing concentrations of KCl-solution as eluant, it was found that the acid-soluble polyphosphate was a mixture of polyphosphates with a variety of condensation number (n-values). Polyphosphates of the n-values between 3 and 15 were only 20% of the total acid-soluble polyphosphate. The majority of the other polyphosphates had greater n-values which was eluded with 0.5-1.0 _M Clue.
- 日本植物生理学会の論文
著者
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Aoki Shigeji
Institute Of Applied Microbiology University Of Tokyo And Tokugawa Institute For Biological Research
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Aoki Shigeji
Institute Cf Applied Microbiology University Of Tokyo And Tokugawa Institute For Biological Research
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Miyachi Shigetoh
Institute Of Applied Microbiology Univcrsity Of Tokyo
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Miyachi Shigetoh
Institutc Of Applied Microbiology University Of Tokyo
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MIYACHI SHIGETOH
Institute of Applied Microbiology, University of Tokyo, and Tokugawa Institute for Biological Research
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