DE- AND RE-GENERATION OF CHLOROPLASTS IN THE CELLS OF CHLORELLA PROTOTHECOIDES IV. EFFECTS OF 5-FLUOROURACIL, DIHYDROSTREPTOMYCIN, CHLORAMPHENICOL AND ACRIDINE ORANGE ON THE PROCESSES OF GREENING AND DIVISION OF "GLUCOSE-BLEACHED" ALGAL CELLS
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概要
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1. The "glucose-bleached" cells of Chlorella protothecoides, which were obtained by the method described previously, were transferred to a glucose-free medium containing basal mineral nutrients alone in the dark, and after a certain period of time, the cell suspension was supplied with urea and light to induce the greening of cells. At different times before and after the provision of urea and light, the inhibitors were applied to the culture to test their effects upon the process of greening. 2. Marked greening of the glucose-bleached cells occurred after a lag period in the control culture. 5-Fluorouracil inhibited the cell greening strongly when it was applied at different times before the provision of urea and light. When applied after the provision of urea and light, the suppressive effect of 5-fluorouracil gradually decreased with the delay of its application. No inhibitive effect was observed when the uracil analogue was added later than the 12th hr after the provision of urea and light, the time around which the chlorophyll formation started in the control culture. On the other hand, the cell division was much more strongly affected by 5-fluorouracil. Even when it was applied at the 18th hr after the provision of urea and light, the cell division was completely halted, indicating that the greening and division of the glucose bleached cells are separate processes. Different mechanisms of action of the uracil analogue towards these two processes were suggested. 3. Dihydrostreptomycin showed its strongest suppressive effect when added at the beginning of the dark incubation of algal cells in the glucose-free medium, and with the delay of application, its effect was progressively reduced, even during the period of the dark incubation. The suppression, however, was still marked when it was applied at the 15th hr. 4. Chloramphenicol was found to inhibit strongly the chlorophyll formation and protein synthesis, but, to a much lesser extent, RNA synthesis. Acridine orange suppressed the cell greening and division at such a low concentration as 1.5 μg/ml. 5. Based on these observations it was concluded that syntheses of nucleic acid and protein are essential processes for the greening of the glucose-bleached algal cells. Successive events occurring in the greening process were discussed.
- 日本植物生理学会の論文
著者
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Hase Eiji
Institute Of Applied Microbiology University Of Tokyo:tokugawa Institute For Biological Research
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Hase Eiji
Institute Applied Microbiology University Of Tokyo:tokugawa Institute For Biological Research
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Aoki Shigeji
Institute Of Applied Microbiology University Of Tokyo:tokugawa Institute For Biological Research
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Aoki Shigeji
Institute Cf Applied Microbiology University Of Tokyo And Tokugawa Institute For Biological Research
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MATUBARA JUNKO
Institute of Applied Microbiology, University of Tokyo
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Matubara Junko
Institute Of Applied Microbiology University Of Tokyo:tokugawa Institute For Biological Research
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