Carbonic Anhydrase from the Blue-Green Alga (Cyanobacterium) Anabaena variabilis

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Carbonic anhydrase (CA) activity was detected in homogenates from Anabaena variabilis ATCC 29413, M-2 and M-3, but not in the suspension of the intact cells. Activity was higher in cells grown in ordinary air (low-CO_2 cells) than in those grown in air enriched with 2-4% CO_2. (high-CO_2 cells). Fractionation by centrifugation indicated that the CA from A. variabilis ATCC 29413 is soluble, whereas both soluble and insoluble forms exist in A. variabilis M-2 and M-3. The addition of dithiothreitol and Mg^<2+> greatly decreased the CA activity of A. variabilis ATCC 29413. The specific activity of the CA from A. variabilis ATCC 29413 was increased ca. 200 times by purification with ammonium sulfate, DEAE-Sephadex A-50 and Sephadex G-100. Major and minor CA peaks in Sephadex G-100 chromatography showed respective molecular weights of 48,000 and 25,000. The molecular weight of the CA determined by polyacrylamide disc gel electrophoresis was 42,000±5,000. The activity of CA was inhibited by ethoxyzolamide (I_<50>=2.8 × 10^<-9> M), acetazolamide (I_<50>=2.5 × 10^<-7> M) and sulfanilamide (I_<50>=2.9 × 10^<-6> M).
日本植物生理学会の論文