Alkalization of the Medium by Unicellular Green Algae during Uptake of Dissolved Inorganic Carbon
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概要
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When Chlorella vulgaris 11h, Chlorella vulgaris C-1, Chlamydomonas reinhardtii. Chlamydomonas moewusii. Scenedesmus obliquus, or Dunaliella tertiolecta were illuminated in water with 0.5 mM NaHCO_3, the pH of the medium increased in a few minutes from 6 to about 9 or 10. The alkalization, which was accompanied by O_2 evolution, was dependent on light, external dissolved inorganic carbon (DIC) as HCO_3^-, and algae grown or adapted to a low, air-level of CO_2 in order to develop a DIC concentrating mechanism. There was little pH increase by algae without a DIC concentrating process from growth on 3% CO_2 in air. Photosynthetic O_2 evolution without alkalization occurred using either internal DIC or external CO_2 at acidic pH. The pH increase stopped between pH 9 to 10, but the alkalization would restart upon re-acidification to between pH 6 and 8. Alkalization was suppressed by the carbonic anhydrase inhibitors, acetazolamide, ethoxyzolamide or carbon oxysulfide. The pH increase appeared to be the consequence of the external conversion of HCO_3^- into CO_2 plus OH^- during photosynthesis by the cells with a high affinity for CO_2 uptake. Cells grown on high CO_2 to suppress the DIC pump, when given low levels of HCO_3^- in the light, acidified the medium from pH 10 to 7. Air adapted Scenedesmus cells with a HCO_3^- pump, as well as a CO_2 pump, alkalized the medium very rapidly in the light to a pH of over 10, as well as slower in the dark or in the light with DCMU or without external DIC and O_2 evolution. Alkalization of the medium during photosynthetic DIC uptake by algae has been considered to be part of the global carbon cycle for converting H_2CO_3 to HCO_3^- and for the formation of carbonate salts by calcareous algae from the alkaline conversion of bicarbonate to carbonate. These processes seem to be a consequence of the algal CO_2 Concentrating process.
著者
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Tolbert N.e.
Department Of Biochemistry Michigan State University
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Goyal Arun
Department Of Biochemistry Michigan State University
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Shiraiwa Yoshihiro
Department Of Biochemistry Michigan State University:(present)department Of Biology Faculty Of Scien
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