STUDIES ON NITELLA HAVING ARTIFICIAL CELL SAP I. REPLACEMENT OF THE CELL SAP WITH ARTIFICIAL SOLUTIONS

元データ 日本植物生理学会

概要

A method for replacing the cell sap of Nitella with an artificial solution was introduced. The technique, which is a modification of KAMIYA and KURODA'S (1, 2), is applicable not only for isotonic but also for hypertonic or hypotonic solutions. Photometric determinations of K^+ Na^+, Ca^<++> and Cl^- proved that the replacement of the cell sap with the present method is satisfactory. The internodal cell of Nitella, whose cell sap was replaced with an isotonic solution with a simple composition such as a mixture of KCl, NaCl and CaCl_2, can be kept living at least for several days, sometimes even for more than one month.

著者

Tazawa Masashi Department Of Applied Physics And Chemistry Fukui University Of Technology
Tazawa Masashi Department Of Biology Faculty Of Scierrce Osaka University
Tazawa Masashi Departlnent Of Biology Faculty Of Science Osaka University
Tazawa M. Department of Biology, Faculty of Scierrce, Osaka University

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