Purification of Actin Based Motor Protein from Chara corallina.
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概要
- 論文の詳細を見る
Actin based motor protein was purified from Chara corallina using high performance liquid chromatography. Sodium dodecyl sulfate gel electrophoresis showed that the molecular weight of the main band was about 230kDa. Although the molecular weight was quite similar to that of the heavy chain of muscle myosin (myosin II), the motor protein was soluble at low ionic strength and antibody raised against the main band did not recognize smooth muscle myosin. The antibody also did not recognize the actin based motor protein from lily pollen tube. The motor protein translocated fluorescently labeled actin filaments at about 25μm/s in the in vitro motility assay. The MgATPase activity of the motor protein was enhanced by F-actin about 150 fold. Calcium ion concentration had little effect on both the motor activity and the actin activated MgATPase activity.
- 日本学士院の論文
著者
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Yamamoto Keiichi
Department Of Biology Chiba University
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Kikuyama Munehiro
Biological Laboratory The University Of The Air
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SUTOH-YAMAMOTO Noriko
Department of Bioengineering, Faculty of Engineering, Soka University
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KAMITSUBO Eiji
Biological Laboratory, Hitotsubashi University
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KIKUYAMA Munehiro
Biological Laboratory, Faculty of Liberal Arts, The University of the Air
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YAMAMOTO Keiichi
Department of Bioengineering, Faculty of Engineering, Soka University
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