Intercellular Transport of Macromolecules in Nitella

元データ 日本植物生理学会

概要

We injected three different fluorescein isothiocyanate (FITC)-labeled proteins, two different FITC-labeled dextrans and the photoprotein aequorin (the molecular weight of each being more than 20 kDa) into internodal cells of Nitella. All macromolecules with molecular weights equal to or less than 45 kDa moved from the injected cell to the neighboring nodal and internodal cells within 24 h after injection. The injected aequorin emitted light in the adjacent internodal cell upon a transient increase in the cytoplasmic concentration of Ca^<2+>, an indication that the aequorin retained its function after transport between cells.

著者

Yamamoto Keiichi Department Of Electronics Faculty Of Engineering Kobe University
Hara Yukichi Department Of Biochemistry Tokyo Medical And Dental University School Of Medicine
Kikuyama Munehiro Biological Laboratory, The University of the Air
Yamamoto K Univ. Of Tsukuba
Shimada Kiyo Biological Laboratory The University Of The Air
Hara Y Tokyo Medical And Dental Univ. School Of Medicine Tokyo Jpn
HIRAMOTO YUKIO Biological Laboratory, University of the Air
Yamamoto K Photonics Research Institute National Institute Of Advanced Industrial Science And Technology (aist)
Hiramoto Yukio Biological Laboratory The University Of The Air
Hara Yohji Biological Laboratory, The University of the Air
Kikuyama Munehiro Biological Laboratory The University Of The Air.
Yamamoto Keiichi Department Of Biology Chiba University
Kikuyama Munehiro Biological Laboratory The University Of The Air
Yamamoto K Saga Univ. Saga Jpn
YAMAMOTO Keiichi Department of Bioengineering, Faculty of Engineering, Soka University

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