Estimation of Cytoplasmic Free Mg^+ Levels and Phosphorylation Potentials in Mung Bean Root Tips by In Vivo ^<31>P NMR Spectroscopy

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概要

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• The cytoplasmic [MgATP]/[ATP]_<free> ratios, free Mg^<2+> concentrations, and phosphorylation potentials in mung bean [Vigna mungo (L.) Hepper] root tip cells were investigated by ^<31>P nuclear magnetic resonance spectroscopy. ^<31>P NMR spectra show well defined peaks due to G6P, cytoplasmic P_i, vacuolar P_i, ATP, UDP-glucose and nicotinamide adenine nucleotides. The concentrations of phosphorus metabolites were determined from quantitative ^<31>P NMR spectra. The [MgATP]/[ATP]_<free> ratio was 9.45. Accordingly, about 90% of the cytoplasmic ATP was complexed to Mg^<2+>. Utilizing the dissociation constant (K_d) determined for MgATP, the cytoplasmic free Mg^<2+> concentration was estimated to be 0.4 mM. The NMR-derived phosphorylation potential, [ATP]/([ADP][P_i]), was 960 M^<-1>. The sodium azide treatment decreased the [ATP]/[ADP] ratio and the phosphorylation potential, and increased the [Mg^<2+>]_<free>. Metabolic inhibi-tion may have been enhanced by an increase in [Mg^<2+>]_<free> and a decrease in the free energy change for ATP hydrolysis, which resulted due to a decrease in the ATP level.

• 日本植物生理学会の論文

著者

• SAKATA Makoto

  Department of Applied Physics, Nagoya University

• Sakata M

  Department Of Applied Physics Nagoya University

• Sakata M

  Kyorin Univ. Tokyo Jpn

• Sakata Makoto

  Department Of Applied Physics Faculty Of Engineering Nagoya University

• OHTA Eiji

  Keio University

• OHTA Eiji

  Department of Material Science, Faculty of Science and Technology, Keio University

• ISHIKAWA Yutaka

  Department of Electro and Electronics Engineering, Nippon Institute of Technology

• Yazaki Yoshiaki

  Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University

• Asukagawa Norio

  Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University

• Ishikawa Yutaka

  Department Of Electrical And Electronics Engineering Nippon Institute Of Technology

• Ohta E

  Keio University

• Ohta Eiji

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• YAZAKI Yoshiaki

  Dept.Plant Physiol., NIAR

• Yazaki Y

  National Inst. Agrobiological Resources Tsukuba Jpn

• Yazaki Yoshiaki

  Department Of Applied Physiology National Institute Of Agrobiological Resources

• Ishikawa Yutaka

  Department Of Instrumentation Engineering Faculty Of Science And Technology Keio University:(present

• Asukagawa Norio

  Department Of Instrumentation Engineering Faculty Of Science And Technology Keio University

• Ohta Eiji

  Department of Instrumentation Engineering,Faculty of Science and Technology,Keio University

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