Membrane Potential Measurement of Protoplasts Isolated from Vigna mungo Hypocotyl Using a Fluorescent Probe, diS-C_3-(5)
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概要
- 論文の詳細を見る
Membrane potentials of protoplasts isolated from Vigna mungo hypocotyl segments were measured using the fluorescent probe diS-C_3-(5). The fluorescence intensity changed in response to the external K^+ concentration. Membrane potential was estimated to be inside negative ( -85±8 mV at O.1 m_M KCl) from the Nernst equation for K^+. The membrane potential was not affected by DCCD (50 μM) or low temperature (5℃). Addition of 0.5 mM Ca^<2+> to the protoplast suspension markedly depolarized the membrane potential, and subsequent EDTA treatment repolarized it to the initial level. The Ca^<2+> effect on the membrane potential may be due to change in the permeability ratio of Cl^- to K^+.
- 日本植物生理学会の論文
著者
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SAKATA Makoto
Department of Applied Physics, Nagoya University
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Sakata M
Department Of Instrumentation Engineering Faculty Of Science And Technology Keio University
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Sakata Makoto
School Of Medicine Kyorin University
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Sakata Makoto
Department Of Applied Physics Faculty Of Engineering Nagoya University
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ISHIKAWA Hideo
Department of Plant Biology, The Ohio State University
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OHTA Eiji
Department of Material Science, Faculty of Science and Technology, Keio University
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Ohta E
Keio Univ. Yokohama Jpn
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OKA Kotaro
Department of Biosciences and Informatics, Keio University
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Yoshida M
Kinki Univ. Nara Jpn
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Ohta Eiji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Oka Kotaro
Department Of System Design Faculty Of Science And Technology Keio University
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Oka Kotaro
Department Of Biosciences And Bioinformatics Faculty Of Science And Technology Keio University
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Sakata M
School Of Medicine Kyorin University
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Oshio Ken-ichi
Department Of Physiology School Of Medicine Kinki University
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Ishikawa Hideo
Department Of Instrumentation Engineering Faculty Of Science And Technology Keio University
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Naitou Shigehiro
Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University
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Yoshida Motoko
Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University
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OHTA Eiji
Mole. Func. Lab., Natl. Food Res. Inst.
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Naitou Shigehiro
Department Of Instrumentation Engineering Faculty Of Science And Technology Keio University
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Ishikawa Hideo
Department Of Botany The Ohio State University
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Ohta Eiji
Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University
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