Effect of Long-Term Shear Stress Exposure on Calcium Response and Morphology of Cultured Endothelial Cells(<Special Issue>Bioengineering)

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Endothelial cells (Ecs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow, in vivo, and shear stress affects the intracellular calcium ([Ca〜<2+>]_1), which initiates cellular responses. Although shearstress induced [Ca^<2+>]_1 responses shortly (within 10 min) after initiation of flow have been studied, the effects of long-term exposure (24 hr) of shear stress on [Ca^<2+>]_1 responses have not. Here, we studied the effect of long-term exposure of shear stress on [Ca^<2+>]_1 responses in cultured Ecs by using a confocal laser microscope and calcium indicator (Calcium Green-1/AM). At the initiation of shear stress of 2 Pa (0 hr), 27% of the cells exhibited [Ca^<2+>]_1 responses. This percentage gradually decreased with increasing exposure time, reaching about 4% after 24 hr of exposure. These data indicate that long-term shear-stress exposure affects [Ca^<2+>]_1 responses in cultured Ecs.
一般社団法人日本機械学会の論文
2003-12-15