KINETICS OF BACTERICIDAL ACTIVITY OF AMINOGLYCOSIDES DURING DYNAMIC DILUTION

概要

論文の詳細を見る
The time courses for viable microorganism count after addition of aminoglycosides were investigated in exponentially decreasing concentrations in in vitro using a continuous flow culture system. When aminoglycosides were added to the incubation medium containing Escherichia coli, the growth rate began to decrease after a lag phase and recovered gradually after the concentration fell below its effective level. To simulate this time course, the following equation including the retardation function was proposed ; [numerical formula] where N is the number of viables, k_o is the generation rate constant, k_d is the dilution rate constant, P_c is the bactericidal coefficient per unit concentration characteristic to the individual antibiotic, k_r is the reciprocal of the retardation time and C_o is the initial concentration of the antibiotic. P_c and k_r were calculated using the nonlinear least square method and the calculated time course agreed with the observed experimental data indicating the appropriateness of this equation. P_c has a negative relationship to the minimum inhibitory concentration for six aminoglycosides studied, kanamycin, amikacin, kanamycin B, tobramycin, dibekacin and habekacin. The values of k_r ranged between 3.12×10^<-2> to 6.40×10^<-2> min^<-1> and are thought to correlate with the mechanism of antibiotic actions.
公益社団法人日本薬学会の論文