1006 フォトクロミック蛍光タンパク質を用いた細胞内物質拡散(S03-1 細胞・生体分子のバイオメカニクス(1),S03 細胞・生体分子のバイオメカニクス)

概要

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In plasma membrane of epithelial cells, immune cells, and other cells called "polarized cell", various molecules are asymmetrically distributed. Polarized cells seemed to control their activity by dynamically polarizing molecules. Especially, when endothelial cells are migrating, some molecules associating with signal transduction are relocalized at leading edge or rear edge. It is clear that polarization have an important role biologically. However, it is not well known how polarization will occur and whether behavior of molecule being localized at different domain, has changed by polarization. To define being differences of molecular property among domains, we visualized molecular diffusion at various polarization domains of cytoplasm, using photocromic fluorescent protein "Dronpa". Then we had indicated that mean diffusion coefficient is D=13.1×10^<-8>cm^2/s, is same value as FRAP. We were able to visualize diffusion at various domains in cytoplasm. There seemed to be differences of diffusion coefficient among various domains.
一般社団法人日本機械学会の論文
2007-09-07