T0201-1-1 せん断応力下における血管内皮細胞細胞膜タンパク質の運動の可視化([T0201-1]細胞の構造と流れにおけるマイクロ・ナノスケール解析(1))

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Many biological procedures in endothelial cell are arranged by shear stress stimulation derived from blood flow. In the past study, the effect of shear stress stimulation on the lipid membrane fluidity is measured and revealed that the effect was variable and non-uniformity. The lipid fluidity modulation seemed to affect the action of membrane molecules. However, it is unclear about the effect of the shear stress on the dynamics of membrane protein. For this reason, we visualize a glycosil phosphatidylinositol anchored protein (GPI-AP) diffusion using photocromic fluorescent protein "Dronpa" (DG-GPI), and estimated the diffusion coefficient D of DGGPI. While 2.5min shear stress stimulation, upstream D_<up> value rose to 1.2-times of D_<up> value under static state(D=0.165±0.013[μm^2/s] (mean±S.E.) (n=27)). And upstream D_<up> was higher than the value of downstream D_<dwn>. These changes were same as the lipid fluidity modulation but were minor.
2010-09-04