Surface Charge Density Estimation of Vigna mungo Protoplasts Using a Fluorescent Dye, 9-Aminoacridine
スポンサーリンク
概要
- 論文の詳細を見る
We showed that the surface charge density of protoplasts can be estimated by the 9-aminoacridine method. The estimated surface charge density of the protoplasts isolated from elongating regions of Vigna mungo root was -39±8 mC/m^2. The negative surface charge density increased when protoplasts were treated with glutaraldehyde or when EDTA was added to the protoplast suspension medium. These results support the validity of our estimation of the surface charge density of protoplasts by the 9-aminoacridine method. The concentration of amino groups at the surface of the protoplasts was estimated to be 34 mC/m^2.
- 日本植物生理学会の論文
著者
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Sakata M
Department Of Instrumentation Engineering Faculty Of Science And Technology Keio University
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Sakata Makoto
School Of Medicine Kyorin University
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Sakata Makoto
Department Of Applied Physics Faculty Of Engineering Nagoya University
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Ohta E
Keio Univ. Yokohama Jpn
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Ohta Eiji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Oka Kotaro
Department Of System Design Faculty Of Science And Technology Keio University
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Oka Kotaro
Department Of Biosciences And Bioinformatics Faculty Of Science And Technology Keio University
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Sakata M
School Of Medicine Kyorin University
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Oshio Ken-ichi
Department Of Physiology School Of Medicine Kinki University
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Ishikawa Hideo
Department Of Instrumentation Engineering Faculty Of Science And Technology Keio University
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OHTA Eiji
Mole. Func. Lab., Natl. Food Res. Inst.
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Ikeshima Hiroko
Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University
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Ishikawa Hideo
Department Of Botany The Ohio State University
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Ohta Eiji
Department of Instrumentation Engineering, Faculty of Science and Technology, Keio University
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